Anti-GRP94 抗体 [SPM249] - BSA and Azide free (ab269787)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab269787)

    Key features and details

    • Rat monoclonal [SPM249] to GRP94 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-GRP94 antibody [SPM249] - BSA and Azide free
      GRP94 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [SPM249] to GRP94 - BSA and Azide free

    • 由来種

      Rat

    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data

    • 免疫原

      Full length native protein (purified) corresponding to Chicken GRP94 aa 1-800. (Purified glucose regulated protein 94 (grp94) from chicken oviducts).
      Database link: P08110

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • IHC-P: Human breast carcinoma tissue.

    • 特記事項

      ab269787 is a carrier free version of ab233979. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.

       

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269787 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    アプリケーション Species
    IHC-P
    Human
    All applications
    Orangutan
    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    (Primary incubation for 30 minutes at Room temperature)

     
    特記事項
    IHC-P
    Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    (Primary incubation for 30 minutes at Room temperature)

     

    ターゲット情報

    • 機能

      Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

    • 配列類似性

      Belongs to the heat shock protein 90 family.

    • 細胞内局在

      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 94 kDa glucose regulated protein antibody
        • 94 kDa glucose-regulated protein antibody
        • ECGP antibody
        • Endoplasmin antibody
        • Endothelial cell (HBMEC) glycoprotein antibody
        • ENPL_HUMAN antibody
        • Glucose regulated protein 94kDa antibody
        • gp96 antibody
        • gp96 homolog antibody
        • GRP 94 antibody
        • GRP-94 antibody
        • Heat shock protein 90 kDa beta member 1 antibody
        • heat shock protein 90kDa beta (Grp94), member 1 antibody
        • Heat shock protein, 90 kDa, beta, 1 antibody
        • HSP90B1 antibody
        • Stress inducible tumor rejection antigen GP96 antibody
        • TRA1 antibody
        • tumor rejection antigen (gp96) 1 antibody
        • Tumor rejection antigen 1 antibody
        • Tumor rejection antigen gp96 antibody
        • Tumor rejection antigen-1 (gp96) antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab269787)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab269787)

        Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for GRP94 using ab233979 at 1 μg/ml in immunohistochemical analysis.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233979).

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269787 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269787 は論文での使用が確認できていません。

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