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  1. Link

    grp94-antibody-ab3674.pdf

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Anti-GRP94 抗体 (ab3674)

  • Datasheet
Reviews (10) Submit a question References (19)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
  • Western blot - Anti-GRP94 antibody (ab3674)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
  • Western blot - Anti-GRP94 antibody (ab3674)

Key features and details

  • Rabbit polyclonal to GRP94
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Recombinant Human GRP94 protein (ab87144)

関連製品

製品の概要

  • 製品名

    Anti-GRP94 antibody
    GRP94 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to GRP94
  • 由来種

    Rabbit
  • 特異性

    At a very high concentration (<1/100), this antibody may recognise PDI and calreticulin. We recommend using this antibody at a relatively high dilution.
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Whole protein from dog pancreas.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GRP94 protein (ab87144)

アプリケーション

Our Abpromise guarantee covers the use of ab3674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/5000. Detects a band of approximately 94 kDa. Can be used at dilutions up to 1/30,000.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview).

ターゲット情報

  • 機能

    Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
  • 配列類似性

    Belongs to the heat shock protein 90 family.
  • 細胞内局在

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P14625 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7184 Human
    • Entrez Gene: 22027 Mouse
    • Omim: 191175 Human
    • SwissProt: P14625 Human
    • SwissProt: P08113 Mouse
    • Unigene: 192374 Human
    • Unigene: 87773 Mouse
    • 別名

      • 94 kDa glucose regulated protein antibody
      • 94 kDa glucose-regulated protein antibody
      • ECGP antibody
      • Endoplasmin antibody
      • Endothelial cell (HBMEC) glycoprotein antibody
      • ENPL_HUMAN antibody
      • Glucose regulated protein 94kDa antibody
      • gp96 antibody
      • gp96 homolog antibody
      • GRP 94 antibody
      • GRP-94 antibody
      • Heat shock protein 90 kDa beta member 1 antibody
      • heat shock protein 90kDa beta (Grp94), member 1 antibody
      • Heat shock protein, 90 kDa, beta, 1 antibody
      • HSP90B1 antibody
      • Stress inducible tumor rejection antigen GP96 antibody
      • TRA1 antibody
      • tumor rejection antigen (gp96) 1 antibody
      • Tumor rejection antigen 1 antibody
      • Tumor rejection antigen gp96 antibody
      • Tumor rejection antigen-1 (gp96) antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).
    • Western blot - Anti-GRP94 antibody (ab3674)
      Western blot - Anti-GRP94 antibody (ab3674)
      Anti-GRP94 antibody (ab3674) at 1/2000 dilution + Cell lysates prepared from mouse fibroblasts


      Western blot of mouse fibroblasts using ab3764 at 1/2000.
    • Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
      Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)This image is courtesy of an anonymous Abreview
      ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)

      Ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    • Western blot - Anti-GRP94 antibody (ab3674)
      Western blot - Anti-GRP94 antibody (ab3674)Image courtesy of an anonymous Abreview.
      All lanes : Anti-GRP94 antibody (ab3674) at 1/2000 dilution

      Lane 1 : Whole cell lysate prepared from murine 3T3 cells
      Lane 2 : Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hours

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit IgG at 1/2000 dilution

      Developed using the ECL technique.

      Observed band size: 94 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds

      See Abreview

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (19)

    ab3674 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3674 は 19 報の論文で使用されています。

    • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
    • Wolf-Dennen K  et al. Exosomal communication by metastatic osteosarcoma cells modulates alveolar macrophages to an M2 tumor-promoting phenotype and inhibits tumoricidal functions. Oncoimmunology 9:1747677 (2020). PubMed: 32313728
    • Shang A  et al. Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1. Aging (Albany NY) 12:8352-8371 (2020). PubMed: 32364530
    • Chiritoiu M  et al. EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired. Int J Mol Sci 21:N/A (2020). PubMed: 32423001
    • Zeng Y  et al. Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis. J Extracell Vesicles 8:1629865 (2019). PubMed: 31258881
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-GRP94 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bonemarrow)
    Specification
    Bonemarrow
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 25 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Fibroblast)
    Specification
    Fibroblast
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1 Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 23 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (3T3)
    Specification
    3T3
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Saponin
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 31 2011

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Macrophages)
    Loading amount
    40 µg
    Specification
    Macrophages
    Treatment
    Thapsigargin for 24 hours
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 30 2011

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3)
    Loading amount
    50 µg
    Specification
    3T3
    Treatment
    2.5 ug/ml Tunicamycin 10 hours
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 28 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293 EBNA)
    Specification
    HEK293 EBNA
    Fixative
    3% Paraformaldehyde
    Permeabilization
    No
    Blocking step
    NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 22 2010

    Western blot abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (lymphnode and DCEK mouse fibroblast cell)
    Loading amount
    30 µg
    Specification
    lymphnode and DCEK mouse fibroblast cell
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100 (10 mins)
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Dr. Dan Tattersall

    Verified customer

    投稿 Nov 10 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GRP94 antibody - ER Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Renal Carcinoma Cell Line)
    Specification
    Renal Carcinoma Cell Line
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4%
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 12 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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