Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
15000 cells
Specification
huh-7
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbig IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Western Lightning Chemiluminescent Rgnt`
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: faint band at >20 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 13 2008