Anti-groEL 抗体 [9A1/2] (ab82592)
Key features and details
- Mouse monoclonal [9A1/2] to groEL
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG1
製品の概要
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製品名
Anti-groEL antibody [9A1/2]
groEL 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9A1/2] to groEL -
由来種
Mouse -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Escherichia coli -
免疫原
Full length protein corresponding to Escherichia coli groEL. Strain K12
Database link: P0A6F5 -
ポジティブ・コントロール
- E. coli lysate.
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特記事項
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
9A1/2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82592の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 1037522 Escherichia coli
- SwissProt: P0A6F5 Escherichia coli
- SwissProt: P0A6F6 Escherichia coli
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別名
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
see all
画像
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All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution
Lane 1 : groEL recombinant E. coli protein
Lane 2 : Hsp60 recombinant human protein (negative control)
Lane 3 : E. coli lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDaThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab82592 は 18 報の論文で使用されています。
- Fang J et al. Mycobacterium tuberculosis Rv0790c inhibits the cellular autophagy at its early stage and facilitates mycobacterial survival. Front Cell Infect Microbiol 12:1014897 (2022). PubMed: 36439208
- Yeom J & Groisman EA Low Cytoplasmic Magnesium Increases the Specificity of the Lon and ClpAP Proteases. J Bacteriol 203:e0014321 (2021). PubMed: 33941609
- Bao SH et al. A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA. Cell Rep 36:109413 (2021). PubMed: 34289355
- Schuller M et al. Molecular basis for DarT ADP-ribosylation of a DNA base. Nature 596:597-602 (2021). PubMed: 34408320
- Iosub IA et al. Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation. Elife 9:N/A (2020). PubMed: 32356726