Key features and details
- Mouse monoclonal [9A1/2] to groEL
- Suitable for: WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG1
製品名Anti-groEL antibody [9A1/2]
groEL 一次抗体 製品一覧
製品の詳細Mouse monoclonal [9A1/2] to groEL
アプリケーション適用あり: WB, IPmore details
種交差性交差種: Escherichia coli
Full length protein corresponding to Escherichia coli groEL.
Database link: P0A6F5
- E. coli lysate.
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
精製度Tissue culture supernatant
特記事項（精製）Purified from TCS.
Our Abpromise guarantee covers the use of ab82592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).|
|IP||Use at an assay dependent concentration.|
関連性The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner.
- 60 kDa chaperonin antibody
- groEL protein antibody
- groL antibody
All lanes : Anti-groEL antibody [9A1/2] (ab82592) at 1/1000 dilution
Lane 1 : groEL recombinant E. coli protein
Lane 2 : Hsp60 recombinant human protein (negative control)
Lane 3 : E. coli lysate
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 57 kDa
This image was generated using the ascites version of the product.
ab82592 は 12 報の論文で使用されています。
- Iosub IA et al. Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation. Elife 9:N/A (2020). PubMed: 32356726
- Khayeka-Wandabwa C et al. Plasma membrane localization of CYP4Z1 and CYP19A1 and the detection of anti-CYP19A1 autoantibodies in humans. Int Immunopharmacol 73:64-71 (2019). PubMed: 31082724
- McHugh RE et al. Characterization of the Mode of Action of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis. Infect Immun 87:N/A (2019). PubMed: 30455200
- Ma B et al. The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-ß-lactamase. Nat Commun 10:3517 (2019). PubMed: 31388008
- Khodaparast L et al. Aggregating sequences that occur in many proteins constitute weak spots of bacterial proteostasis. Nat Commun 9:866 (2018). PubMed: 29491361