Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4902] to GRO alpha+GRO gamma+GRO beta - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品名Anti-GRO alpha+GRO gamma+GRO beta antibody [EPR4902] - BSA and Azide free
GRO alpha+GRO gamma+GRO beta 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4902] to GRO alpha+GRO gamma+GRO beta - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP
Synthetic peptide within Human GRO alpha+GRO gamma+GRO beta aa 50-150. The exact sequence is proprietary.
ab248048 is the carrier-free version of ab124898 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248048 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 8 kDa (predicted molecular weight: 11 kDa).|
細胞内局在GRO alpha: Secreted. GRO gamma: Secreted.
All lanes : Anti-GRO alpha + GRO gamma + GRO beta antibody [EPR4902] (ab124898) at 1/2000 dilution
Lane 1 : Human GRO alpha active protein
Lane 2 : Human GRO beta fragment recombinant protein
Lane 3 : Human GRO gamma fragment recombinant protein
Lysates/proteins at 10 µg per lane.
Lanes 1 & 3 : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Lane 2 : Goat Ant-Rabbit IgG,(H+L),Peroxidase conjugated at 1/100000 dilution
Predicted band size: 11 kDa
Observed band size: 10,10,36 kDa why is the actual band size different from the predicted?
This data was developed using ab124898, the same antibody clone in a different buffer formulation.
Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
Human GRO beta fragment pritein contain aa35-107 with a GST-Tage. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.