Anti-GRK2 (phospho S29) 抗体 (ab58520)
Key features and details
- Rabbit polyclonal to GRK2 (phospho S29)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GRK2 (phospho S29) antibody
GRK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GRK2 (phospho S29) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human GRK2 aa 1-100 (phospho S29).
Database link: P25098 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58520の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 80 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 80 kDa. |
ターゲット情報
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機能
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
組織特異性
Expressed in peripheral blood leukocytes. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
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参照データベース
- Entrez Gene: 156 Human
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P25098 Human
- SwissProt: P26817 Rat
- Unigene: 83636 Human
- Unigene: 13010 Rat
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別名
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
画像
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All lanes : Anti-GRK2 (phospho S29) antibody (ab58520)
Lane 1 : Jurkat cells treated with EGF
Lane 2 : Jurkat cells treated with EGF with phospho peptide
Predicted band size: 80 kDa -
This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab58520 は 1 報の論文で使用されています。
- Schreckenberg R et al. Adverse Effects on ß-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function. J Am Heart Assoc 6:N/A (2017). PubMed: 29273639