Anti-GRK2 抗体 (ab228705)
Key features and details
- Rabbit polyclonal to GRK2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-GRK2 antibody
GRK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GRK2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Recombinant fragment within Human GRK2 (internal sequence). The exact sequence is proprietary.
Database link: P25098 -
ポジティブ・コントロール
- WB: MOLT-4 whole cell lysate. IHC-P: Mouse brain stem tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228705の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
-
機能
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. -
組織特異性
Expressed in peripheral blood leukocytes. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 282682 Cow
- Entrez Gene: 156 Human
- Entrez Gene: 100513842 Pig
- Entrez Gene: 25238 Rat
- Omim: 109635 Human
- SwissProt: P21146 Cow
- SwissProt: P25098 Human
- SwissProt: Q99MK8 Mouse
see all -
別名
- ADRBK1 antibody
- Adrenergic beta receptor kinase 1 antibody
- ARBK1_HUMAN antibody
see all
画像
-
Anti-GRK2 antibody (ab228705) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 80 kDa7.5% SDS-PAGE
-
Paraffin-embedded mouse brain stem tissue stained for GRK2 with ab228705 at 1/500 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab228705 は 1 報の論文で使用されています。
- Zhang M et al. CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells. Mol Med Rep 20:4831-4842 (2019). PubMed: 31661133