Anti-GRK1 抗体 [G8] (ab2775)

Submit a review Q&A (1)References (2)
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Key features and details

  • Mouse monoclonal [G8] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-GRK1 antibody [G8]
    GRK1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [G8] to GRK1

  • 由来種

    Mouse

  • 特異性

    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Mouse, Cow, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • エピトープ

    This antibody recognizes an epitope in the C-terminal region of GRK1a.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2775の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/200.
特記事項
ICC/IF
1/100 - 1/200.

ターゲット情報

  • 機能

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

  • 組織特異性

    Retinal-specific. Expressed in rods and cones cells.

  • 関連疾患

    Night blindness, congenital stationary, Oguchi type 2

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.

  • 翻訳後修飾

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunolocalization of GRK1 in bovine retina using ab2775.

参考文献 (2)

ab2775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2775 は 2 報の論文で使用されています。

  • Wong BH  et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
  • Aguilà M  et al. Hsp90 inhibition protects against inherited retinal degeneration. Hum Mol Genet 23:2164-75 (2014). PubMed: 24301679

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Customer would like to know if this antibody has specifically been tested with total eye extracts from rat or mouse.

Read More
Answer

This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additional questions, please contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com