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Anti-GRK1 抗体 [G8] (ab2775)

Submit a review Q&A (1)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

Key features and details

  • Mouse monoclonal [G8] to GRK1
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-GRK1 antibody [G8]
    GRK1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [G8] to GRK1

  • 由来種

    Mouse

  • 特異性

    Detects G-protein-associated rhodopsin kinase 1a (GRK1a).

  • アプリケーション

    適用あり: ICC/IFmore details

  • 種交差性

    交差種: Mouse, Cow, Human

  • 免疫原

    Full length native protein (purified) corresponding to Human GRK1.

  • エピトープ

    This antibody recognizes an epitope in the C-terminal region of GRK1a.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2775の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/200.
特記事項
ICC/IF
1/100 - 1/200.

ターゲット情報

  • 機能

    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.

  • 組織特異性

    Retinal-specific. Expressed in rods and cones cells.

  • 関連疾患

    Night blindness, congenital stationary, Oguchi type 2

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.

  • 翻訳後修飾

    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EC=2.7.11.14 antibody
    • G protein-coupled receptor kinase 1 antibody
    • GPRK1 antibody
    • Grk1 antibody
    • Rhodopsin kinase antibody
    • RHOK antibody
    • RK antibody
    • RK_HUMAN antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2775 (1:100) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)
    Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [G8] (ab2775)

    Immunolocalization of GRK1 in bovine retina using ab2775.

参考文献 (3)

ab2775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2775 は 3 報の論文で使用されています。

  • Zhang X  et al. Disease mechanisms and neuroprotection by tauroursodeoxycholic acid in Rpgr knockout mice. J Cell Physiol 234:18801-18812 (2019). PubMed: 30924157
  • Wong BH  et al. Mfsd2a Is a Transporter for the Essential ?-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem 291:10501-14 (2016). PubMed: 27008858
  • Aguilà M  et al. Hsp90 inhibition protects against inherited retinal degeneration. Hum Mol Genet 23:2164-75 (2014). PubMed: 24301679

レビューと Q&A

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レビューを送る 質問を送る

Question

Customer would like to know if this antibody has specifically been tested with total eye extracts from rat or mouse.

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Answer

This antibody has not specifically been tested with total eye extracts. However, it has been proven to work in both rat and mouse cells in the the following applications: Western blot, Immunoprecipitation, Immunofluorescence. If you have any additional questions, please contact us again.

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