Griess reagent (ab234044)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 1 nmol/well
製品の概要
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製品名
Griess reagent -
検出方法
Colorimetric -
サンプルの種類
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
アッセイタイプ
Quantitative -
検出感度
1 nmol/well -
全工程の試験時間
1h 30m -
製品の概要
Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.
The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K544 Nitrite Assay Kit (Griess Reagent). K544-200 is the same size as the 200 test size of ab234044.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 200 tests 500 tests Buffer III 1 x 30ml 1 x 75ml Griess Reagent I 1 x 10ml 1 x 25ml Griess Reagent II 1 x 10ml 1 x 25ml Nitrite Standard 1 vial 1 vial
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab234044 は 5 報の論文で使用されています。
- Patel B et al. Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain. Mol Metab 43:101123 (2021). PubMed: 33227495
- Varghese MV et al. Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 320:L508-L521 (2021). PubMed: 33502933
- Lee W et al. The Decursin Analog, CYJ-27, Suppresses Inflammation Via the Downregulation of NF-?B and STAT-1. J Med Food 24:852-859 (2021). PubMed: 34382871
- Barmaki S et al. A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture. Micromachines (Basel) 11:N/A (2020). PubMed: 33143339
- Liu Y et al. Lactoferrin-induced myeloid-derived suppressor cell therapy attenuates pathologic inflammatory conditions in newborn mice. J Clin Invest 129:4261-4275 (2019). PubMed: 31483289