Anti-GREB1 抗体 (ab270741)
Key features and details
- Rabbit polyclonal to GREB1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GREB1 antibody
GREB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GREB1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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免疫原
Recombinant fragment corresponding to Human GREB1 aa 600-750.
Database link: Q4ZG55 -
ポジティブ･コントロール
- IHC-P: Human endometrium, placenta, kidney and skin tissue. ICC/IF: MCF7 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Used the PrEST antigen as affinity ligand. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
Unknown. Primary target for estrogen receptor regulation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers. -
組織特異性
Expressed in proliferating prostatic tissue and prostate cancer. -
配列類似性
Belongs to the GREB1 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9687 Human
- Omim: 611736 Human
- SwissProt: Q4ZG55 Human
- Unigene: 467733 Human
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別名
- 5730583K22Rik antibody
- 9130004E13 antibody
- AF180470 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-GREB1 antibody (ab270741)
Immunofluorescence analysis of human MCF7 cells labeling GREB1 using ab270741 at 2 µg/ml (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab270741)Paraffin-embedded human kidney tissue stained for GREB1 using ab270741 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab270741)Paraffin-embedded human placenta tissue stained for GREB1 using ab270741 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab270741)Paraffin-embedded human endometrium tissue stained for GREB1 using ab270741 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GREB1 antibody (ab270741)Paraffin-embedded human skin tissue stained for GREB1 using ab270741 at 1/500 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab270741 は論文での使用が確認できていません。