Anti-GRB2 抗体 (ab2234)
Key features and details
- Goat polyclonal to GRB2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-GRB2 antibody
GRB2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GRB2 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human GRB2 aa 206-217 (C terminal).
Sequence:C-PRNYVTPVNRNV
(Peptide available asab22873) -
ポジティブ･コントロール
- Recombinant Human GRB2 protein (ab85917) can be used as a positive control in WB. WB: MOLT4 cell lysate, mouse, rat, human and pig spleen, mouse and rat brain, and human thymus tissue lysates. IHC-P: Human lymph node tissue.
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特記事項
Principal Names - GRB2; growth factor receptor-bound protein 2 Official Gene Symbol - GRB2. GenBank Accession Number – NP_002077. LocusLink ID - 2885. Gene Ontology terms - cell-cell signalling; SH3/SH2 adaptor protein; EGF receptor signalling pathway; RAS protein signal transduction; cell shape and cell size control.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. |
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| WB | Use a concentration of 0.05 - 0.15 µg/ml. Predicted molecular weight: 30 kDa. A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. -
配列類似性
Belongs to the GRB2/sem-5/DRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The SH3 domains mediate interaction with RALGPS1 and SHB. -
細胞内局在
Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 535298 Cow
- Entrez Gene: 483312 Dog
- Entrez Gene: 2885 Human
- Entrez Gene: 14784 Mouse
- Entrez Gene: 100192436 Pig
- Entrez Gene: 81504 Rat
- Omim: 108355 Human
- SwissProt: P62993 Human
see all -
別名
- Abundant SRC homology antibody
- Adapter protein GRB2 antibody
- ASH antibody
see all
画像
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Mouse brain tissue lysate in RIPA buffer
Lane 2 : Rat brain tissue lysate in RIPA buffer
Lane 3 : Mouse spleen tissue lysate in RIPA buffer
Lane 4 : Rat spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 (1µg/ml) staining of paraffin embedded Human Lymph Node tissue. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody (ab2234)ab2234 negative control showing staining of paraffin embedded Human Lymph Node tissue, with no primary antibody.
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Western blot - Anti-GRB2 antibody (ab2234)All lanes : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 1 : Human spleen tissue lysate in RIPA buffer
Lane 2 : Pig spleen tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
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Western blot - Anti-GRB2 antibody (ab2234)Lane 1 : Anti-GRB2 antibody (ab2234) at 0.05 µg/ml
Lane 2 : Anti-GRB2 antibody (ab2234) at 0.05 µg
Lane 1 : Human thymus tissue lysate in RIPA buffer
Lane 2 : MOLT4 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected using chemiluminescence.
プロトコール
データシートおよび資料
参考文献 (1)
ab2234 は 1 報の論文で使用されています。
- Debattisti V et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep 29:1274-1286.e6 (2019). PubMed: 31665639
