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Anti-GPCR GPR64 抗体 [EPR23658-2] - BSA and Azide free (ab272395)

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Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
  • Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23658-2] to GPCR GPR64 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free
    GPCR GPR64 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23658-2] to GPCR GPR64 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: and A673, A673 whole treated with PNGase F lysates. IHC-P: Human Ewing sarcoma, Mouse epididymis, Rat epididymis, Human leiomyosarcoma and Mouse liver, Rat cerebrum tissues. ICC/IF: A-673 cell.

  • 特記事項

    ab272395 is the carrier-free version of ab272390. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Rat
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 112 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-Fr or IP.

    • ターゲット情報

    • 関連性

      This orphan B G-protein coupled receptor could be involved in a signal transduction pathway controlling epidymal function and male fertility: it has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle and testis libraries.

    • 細胞内局在

      Cell membrane; Multi pass membrane protein.

    • 参照データベース

      see all

    • 別名

      • Epididymis specific protein 6 antibody
      • FLJ00282 antibody
      • G protein coupled receptor 64 antibody
      • G protein coupled receptor epididymis specific antibody
      • GPR 64 antibody
      • GPR64 antibody
      • HE 6 antibody
      • He 6 receptor antibody
      • HE6 antibody
      • He6 receptor antibody
      • MGC104454 antibody
      • MGC138738 antibody
      • MGC138739 antibody
      • TM7LN2 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-673 cells labelling GPCR GPR64 with ab272390 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in A-673 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in rat cerebrum(PMID:12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in mouse liver (PMID:12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Human leiomyosarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human leiomyosarcoma (PMID:23338946).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Rat epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat epididymis (PMID:27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Mouse epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse epididymis (PMID:27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

      Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human Ewing sarcoma (PMID:23338946). The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272390).

    • Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)
      Anti-GPCR GPR64 antibody [EPR23658-2] - BSA and Azide free (ab272395)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272395 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272395 は論文での使用が確認できていません。

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