Anti-GPCR GPR64 抗体 [EPR23658-2] (ab272390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23658-2] to GPCR GPR64
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GPCR GPR64 antibody [EPR23658-2]
GPCR GPR64 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23658-2] to GPCR GPR64 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details
適用なし: Flow Cyt,IHC-Fr or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A673 cell lysate and A673 cell lysate treated with PNGase F. IHC-P: Human Ewing sarcoma, Mouse epididymis, Rat epididymis, Human leiomyosarcoma and Mouse liver, Rat cerebrum tissues. ICC/IF: A-673 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23658-2 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272390の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 112 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/4000.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 112 kDa. |
ICC/IF
1/100. |
IHC-P
1/4000. |
ターゲット情報
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関連性
This orphan B G-protein coupled receptor could be involved in a signal transduction pathway controlling epidymal function and male fertility: it has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle and testis libraries. -
細胞内局在
Cell membrane; Multi pass membrane protein. -
参照データベース
- Entrez Gene: 10149 Human
- Entrez Gene: 237175 Mouse
- Entrez Gene: 266735 Rat
- Omim: 602657 Human
- SwissProt: Q8IZP9 Human
- SwissProt: Q8CJ12 Mouse
- SwissProt: Q8CJ11 Rat
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別名
- Epididymis specific protein 6 antibody
- FLJ00282 antibody
- G protein coupled receptor 64 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Mouse epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse epididymis (PMID:27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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All lanes : Anti-GPCR GPR64 antibody [EPR23658-2] (ab272390) at 1/1000 dilution
Lane 1 : A673 (human muscle Ewing's Sarcoma), whole cell lysate
Lane 2 : A673 whole cell lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 105,130,180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed molecular weight is consistent with what has been described in the literature (PMID: 12420295, 29393851).
Two bands of 180 and 130 KDa in lane 1 are glycosylated forms. The bands around 105KDa in lane 2 are de-glycosylated and partially de-glycosylated forms.
Exposure time: 54 seconds
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-673 cells labelling GPCR GPR64 with ab272390 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in A-673 cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in rat cerebrum(PMID:12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human Ewing sarcoma (PMID:23338946). The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in mouse liver (PMID:12420295).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Human leiomyosarcoma tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human leiomyosarcoma (PMID:23338946).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
Immunohistochemical analysis of paraffin-embedded Rat epididymis tissue labeling GPCR GPR64 with ab272390 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat epididymis (PMID:27476656, 12420295, 18469038).The section was incubated with ab272390 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab272390 は論文での使用が確認できていません。