Application
Western blot
Sample
Human Cell lysate - whole cell (colon cancer cell)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Treatment
gene transfection
Specification
colon cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBST
Dilution
1/1000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: thermo goat anti-rabbit IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 78 kDa Non-specific: 50, >250 kDa
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 20 2020