Goat Anti-Syrian Hamster IgG H&L (HRP) preadsorbed (ab7146)

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Key features and details

  • Goat Anti-Syrian Hamster IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: Dot blot, IHC-P, ICC/IF, Immunomicroscopy, WB, IHC-Fr, ELISA

製品の概要

  • 製品名

    Goat Anti-Syrian Hamster IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Syrian hamster

  • アプリケーション

    適用あり: Dot blot, IHC-P, ICC/IF, Immunomicroscopy, WB, IHC-Fr, ELISAmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    hamster IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.60
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/15000 - 1/75000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7146 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7146 は 1 報の論文で使用されています。

  • Miezeiewski M  et al. An in vitro culture model to study the dynamics of colonic microbiota in Syrian golden hamsters and their susceptibility to infection with Clostridium difficile. ISME J N/A:N/A (2014). PubMed: 25036923

レビューと Q&A

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Question

I did try the primary with a different secondary and the results were beautiful.  I also tried dilutions of the secondary as low as 1:50 to see if I could see any staining.  Furthermore I tried the secondary ab7146 at 1:1000 on a Western and got the cleanest (no bands at all) Western blot I have ever seen.  I think that something is wrong with the secondary.  Could you replace it with Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (HRP) (ab6892)?  Or do you suggest something else?  I only suggest this one instead because it has a reference associated with it.    While I have your ear, I have been meaning to email you about another issue.  We received a new lyve-1 antibody ab14947 on Sept 29, 2011 and we have not had any success using our established protocol.  The established protocol was worked up on lot *** and utilized to stain over 100 slides.  When the new lot, ****, the protocol stopped working.  We have tried different dilutions and we still have no stain.  Furthermore, all other reagents are fine as they have been utilized with different primaries and positive results obtained.  Have you received any other complaints about this lot, and is it possible there may be something wrong?  Do you have any of the other lot available?  Can you replace with a new lot?

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Answer

Thank you for the followup. I agree this needs to be replaced. The antibody you have picked as a replacement, ab6892, is available, but it is not pre-adsorbed. (Ab7146 is pre-adsorbed, to remove reactivity with other species of IgG). Will this be acceptable? It is usually only a requirement if one is staining for multiple targets in a single sample with multiple antibodies. There have not been other complaints about ab14917. We have two more vials of lot **** but they may move quickly. Can you please send a few details of your  IHC protocol (fixation, retrieval, diilution) and samples? Given that it was working with lot ****, we expect it would work with the most recent, but if you are working with a species other than mouse, there might be some lot-to-lot variability with respect to cross-reactivity.

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Question

Will the antibody ab7146 cross react with Armenian Hamster IgG?

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Answer

The antibody ab7146 will not work very well with Armenian Hamster IgG and I have been informed that the most suitable antibody is ab5745 (Rabbit polyclonal to Hamster (Armenian) IgG H&L (HRP)) https://www.abcam.com/index.html?datasheet=5745 I hope this information will help you, please do not hesitate to contact me if I can be of further assistance,

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