Goat Anti-Syrian Hamster IgG H&L (HRP) (ab6892)

Submit a review Q&A (2)References (4)

Key features and details

  • Goat Anti-Syrian Hamster IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

製品の概要

  • 製品名

    Goat Anti-Syrian Hamster IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Syrian hamster

  • アプリケーション

    適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

  • 免疫原

    Full length native hamster IgG (purified).

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Horseradish Peroxidase (HRP)

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent dilution.
ELISA 1/7000 - 1/35000.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/1000 - 1/5000.
ICC/IF 1/500 - 1/2500.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab6892 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6892 は 4 報の論文で使用されています。

  • Walpita P  et al. A VLP-based vaccine provides complete protection against Nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model. NPJ Vaccines 2:21 (2017). PubMed: 29263876
  • Sharma G  et al. Purification and characterization of adenovirus core protein VII: a histone-like protein that is critical for adenovirus core formation. J Gen Virol 98:1785-1794 (2017). PubMed: 28695796
  • Huang Y  et al. Identification of novel genes associated with renal tertiary lymphoid organ formation in aging mice. PLoS One 9:e91850 (2014). PubMed: 24637805
  • Hoffhines AJ  et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096-105 (2009). PubMed: 19047058

レビューと Q&A

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Question

We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we checked your website information it is recommended to be kept in 4 degree castigates.

Please let me know if keeping them in -20 C0 has made any problem for them and can we continue to keep them in this temperature or not?

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Goat-polyclonal-Secondary-Antibody-to-Horse-IgG-H-L-HRP(ab102396)

Goat-polyclonal-Secondary-Antibody-to-Syrian-Hamster-IgG-H-L(HRP-ab6892)

Goat-polyclonal-Secondary-Antibody-to-Human-IgM-mu-chain-HRP(ab97205)

Goat-polyclonal-Secondary-Antibody-to-Cow-IgG-H-L-HRP(ab6934)

Rabbit-polyclonal-Secondary-Antibody-to-Sheep-IgG-Fc-HRP (ab102299)

Rabbit-polyclonal-Secondary-Antibody-to-Goat-IgG-Fc-HRP-pre-adsorbed (ab102345)

Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-HRP (ab97265)

Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-Fc-HRP-pre-adsorbed (ab97090)


Best,

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to find out which species, Armenian or Syrian, of hamster　the Goat anti-Hamster IgG HRP (product code ab6892) is from. I could not　find this information on the data sheet provided. Thank you,

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Answer

Thank you for your enquiry. I can confirm that the immunogen used for this product is Golden Syrian Hamster IgG. Please do not hesitate to contact me should you need any further information.

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