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Goat Anti-Syrian hamster IgG H&L (Alkaline Phosphatase) (ab102305)

Submit a review Q&A (1)

Key features and details

  • Goat Anti-Syrian hamster IgG H&L (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ICC, ELISA, WB

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 APC Biotin Cy3 ® DyLight® 488 FITC HRP PE/Cy7® Unconjugated Unconjugated

製品の概要

  • 製品名

    Goat Anti-Syrian hamster IgG H&L (Alkaline Phosphatase)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Syrian hamster

  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with syrian hamster IgG and with light chains common to other syrian hamster immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

  • アプリケーション

    適用あり: IHC-P, ICC, ELISA, WBmore details

  • 標識

    Alkaline Phosphatase

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab102305の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100 - 1/1000.
ICC
1/100 - 1/1000.
ELISA
1/1000 - 1/10000. (Primary).
WB
1/1000 - 1/10000. (Chemiluminescent).
特記事項
IHC-P
1/100 - 1/1000.
ICC
1/100 - 1/1000.
ELISA
1/1000 - 1/10000. (Primary).
WB
1/1000 - 1/10000. (Chemiluminescent).

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab102305 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab102305 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Question

Could you please explain what is a pre-adsorbed antibody? During an ELISA experiment what is the difference between using the ab102305 and the ab102315? Thank you in advance for your attention to this matter.

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Answer

Thank you for contacting us.

"Pre-adsorbed" refers to purification of the secondary antibody that removes any antibodies in the preparation that might react with immunoglobulins of species other than Syrian Hamster.

The secondary antibodies ab102305 and ab102315 are the same, except that ab102315 has the extra purification step. In the field on the datasheets titled "Purification Notes", you will see these notes:

ab102305

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.

ab102315

Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase.

The antibody that is cpre-adsorbed, ab102315, is the better choice if your ELISA is a sandwich ELISA, since you do not want the secondary antibody to react with the capture antibody coated onto the plate. It is likely that the capture antibody will be from a mouse or rabbit. (I am also assuming that your samples themselves are not Syrian hamster lysates).

If you are doing an indirect ELISA, coating the sample onto the plate and detecting with a Syrian hamster antibody, then either secondary antibody will be suitable, assuming your samples have no immunoglobulins in them. If they do, the secondary antibody ab102305 might detect them, since it has not been pre-adsorbed to remove reactivity with IgG in human and mouse samples, among other species.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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