Goat anti-Rat IgG H&L (PE) Pre-Adsorbed (ab7010)
Key features and details
- Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt
製品の概要
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製品名
Goat anti-Rat IgG H&L (PE) Pre-Adsorbed
IgG 二次抗体 製品一覧 -
製品の詳細
Goat anti-Rat IgG H&L (PE) Pre-Adsorbed -
由来種
Goat -
ターゲット生物種
Rat -
アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details -
吸着処理血清
Cow, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Full length native protein (purified) corresponding to IgG.
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標識
PE. Ex: 488nm, Em: 575nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
特記事項（標識）
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab7010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Immunomicroscopy | 1/50 - 1/200. | |
| Flow Cyt | 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (19)
ab7010 は 19 報の論文で使用されています。
- Yang Y et al. 1-Trichloromethyl-1,2,3,4-tetrahydro-beta-carboline (TaClo) Induces the Apoptosis of Dopaminergic Neurons via Oxidative Stress and Neuroinflammation. Oxid Med Cell Longev 2019:1292891 (2019). PubMed: 30984332
- Zeng L et al. Long non-coding RNA MALAT-1 contributes to maintenance of stem cell-like phenotypes in breast cancer cells. Oncol Lett 15:2117-2122 (2018). PubMed: 29434914
- Lin L et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). PubMed: 30066844
- Cao Y et al. Isoflurane-induced postoperative cognitive dysfunction is mediated by hypoxia-inducible factor-1a-dependent neuroinflammation in aged rats. Mol Med Rep 17:7730-7736 (2018). PubMed: 29620198
- Lei X et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). PubMed: 29693708
