Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
Key features and details
- Goat Anti-Rat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blot
製品の概要
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製品名
Goat Anti-Rat IgG H&L (HRP) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
免疫原
Full length native Rat IgG (purified).
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
Our Abpromise guarantee covers the use of ab7097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/500 - 1/1500. | |
IHC-P | Use at an assay dependent dilution. | |
IHC-Fr | Use at an assay dependent dilution. | |
WB | Use at an assay dependent dilution. | |
ELISA | 1/50000. | |
Dot blot | Use at an assay dependent dilution. |
画像
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Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution + Rat IgG, 50 ng
Both the heavy and light chains of rat IgG are detected.
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IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)This picture was kindly supplied as part of the review submitted by Helen Makarenkova.
Whole mount immunostaining of E11.5 mouse embryo (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab7097 (HRP conjugated).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (20)
ab7097 は 20 報の論文で使用されています。
- Luo P et al. Divergent Roles of Kupffer Cell TLR2/3 Signaling in Alcoholic Liver Disease and the Protective Role of EGCG. Cell Mol Gastroenterol Hepatol 9:145-160 (2020). PubMed: 31562937
- Liu Y et al. CD28 Deficiency Ameliorates Blast Exposure-Induced Lung Inflammation, Oxidative Stress, Apoptosis, and T Cell Accumulation in the Lungs via the PI3K/Akt/FoxO1 Signaling Pathway. Oxid Med Cell Longev 2019:4848560 (2019). PubMed: 31565151
- Zhang Y et al. Effects of resveratrol on learning and memory in rats with vascular dementia. Mol Med Rep 20:4587-4593 (2019). PubMed: 31702039
- Kenyon E et al. Ductal tree ablation by local delivery of ethanol prevents tumor formation in an aggressive mouse model of breast cancer. Breast Cancer Res 21:129 (2019). PubMed: 31779648
- Materna SC et al. Cardiovascular development and survival require Mef2c function in the myocardial but not the endothelial lineage. Dev Biol 445:170-177 (2019). PubMed: 30521808