Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Paraffin-embedded mouse spleen:
Section was deparaffinized
Antigen retrieval Dako, pH6 for 15' in microwave
Peroxidase block Dako 10'
protein block Dako 10'
Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
Goat anti-rat IgG PolymerHRP 20' RT
Colour developed with DAB
Counterstained with haematoxylin and mounted
Section was deparaffinized
Antigen retrieval Dako, pH6 for 15' in microwave
Peroxidase block Dako 10'
protein block Dako 10'
Anti-mouse B220 (Biolegend, 0.5 mg/ml, 1:100) 30' RT
Goat anti-rat IgG PolymerHRP 20' RT
Colour developed with DAB
Counterstained with haematoxylin and mounted
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Davide Lucchesi
Verified customer
投稿 Aug 01 2017