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  1. Link

    goat-rat-igg-hl-cy5--preadsorbed-ab6565.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (21)

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)

    Key features and details

    • Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
    • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
    • Host species: Goat
    • Isotype: IgG
    • Suitable for: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 6nm HRP HRP polymer PE PE Texas Red ® TRITC Unconjugated Unconjugated

    こちらの製品もご検討ください

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    ブロッキング
    Product image
    Normal Goat Serum (ab7481)

    関連製品

    製品の概要

    • 製品名

      Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed
      IgG 二次抗体 製品一覧
    • 由来種

      Goat
    • ターゲット生物種

      Rat
    • アプリケーション

      適用あり: ICC, Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
    • 吸着処理血清


      Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • 免疫原

      Rat IgG, whole molecule (H&L)

    • 標識

      Cy5 ®. Ex: 650nm, Em: 667nm

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C.
    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    • 特記事項(標識)

      Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 特記事項

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


      This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

    • 研究分野

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Rat
      • IgG
      • Fluorophore
      • Cy5

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6565の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    特記事項
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent dilution.
    ICC/IF
    1/1000 - 1/5000.
    ELISA
    1/10000 - 1/50000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.

    画像

    • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)
      Immunocytochemistry/ Immunofluorescence - Goat Anti-Rat IgG H&L (Cy5 ®) preadsorbed (ab6565)

      ab6565 was used at dilution 1/500 with the primary antibody ab6161 in ICC/IF. See the review on ab6161.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (21)

    ab6565 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6565 は 21 報の論文で使用されています。

    • Ejaz M  et al. ARABIDOPSIS THALIANA HOMEOBOX GENE 1 controls plant architecture by locally restricting environmental responses. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33888582
    • Baxley RM  et al. Bi-allelic MCM10 variants associated with immune dysfunction and cardiomyopathy cause telomere shortening. Nat Commun 12:1626 (2021). PubMed: 33712616
    • Ferreira P  et al. MTBP phosphorylation controls DNA replication origin firing. Sci Rep 11:4242 (2021). PubMed: 33608586
    • Li Q  et al. Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin. Theranostics 11:6461-6476 (2021). PubMed: 33995668
    • Fu H  et al. Dynamics of replication origin over-activation. Nat Commun 12:3448 (2021). PubMed: 34103496
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

     Dear technical support: This customer has highly interested in ab6565 Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5 ®), pre-adsorbed) and ab6346 (Anti-Tenascin C antibody [MTn-12]), this customer would like to use these antibodies to conduct ELISA assay. Therefore, he wants to ask for your help to offer him any suggestion (e.g. blocking buffer and washing Buffer and protocol etc…), would you please help this customer to solve the problem? Thanks for your kindly assistance. Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2011

    Answer

    Thank you very much for your interest in our products. I can confirm that ab6346 is tested and guaranteed for ELISA whereas ab6565 is not yet tested. Please find a testing discount offer below for ab6565. I attached protocols for indirect and direct ELISA for the information of your customer. These protocols and more can also be found using this link: https://www.abcam.com/index.html?pageconfig=popular_protocols   I hope this information is helpful. Please do not hesitate to contact me again with any further questions. If you do, could you please include more information about the planned experiments of your customer? This will make it easier for me to help your customer with more specific suggestions.   Testing Discount Offer: To our knowledge, ab6565 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab6565 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free secondary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab6565 in ELISA. I will then send a discount code. This code must be issued before purchasing ab6565 so please wait for my reply before ordering. 2. Purchase ab6565 either by phone, fax, or online (www.abcam.com). 3. Test it in ELISA. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any secondary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab6565 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

    Read More

    Abcam Scientific Support

    Answered on Oct 31 2011

    Question

    In the title of the attchament I said I used Cell cultures from bEnd3 cells. Yes, I though that the problem could be the secondary antibody but I bought it to abcam at the same time (the problem is the same) and to clarify this issue I need another primary of the same specie (rat) because I don't have it to test.   Thanks in advance,  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an alternative antibody which worked fine with these cells. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.   I could raise either a credit note for the cost of  one vial of ab7388 and ab7388 since you have alternative antibodies; or send you two replacement vials (one of each)  free of charge. Please could you let me know how you wish to proceed with your enquiry? I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2011

    Question

    I send in attach the protocol I used (that unswer to all questions of the inquiry). This protocol is optimized for a lot of antigens, namely CD31....   Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI. Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI.  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 19 2011

    Answer

    Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used for the staining i.e. species, cells (cultured) or tissues? 2) Detection system: Have you used the secondary antibody successfully with another primary antibody? Do you think that the problem may come from the secondary antibody? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Abcam Scientific Support

    Answered on Oct 19 2011

    Question

    Hi! For experiment design, I want to know if this antibody would cross react with mouse IgG? I couldn't fine the reactivity information of this Ab in your company's website. Is there any information about this? Very thanks for your help!!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2010

    Answer

    Thank you for your enquiry. I can confirm that ab6565 Goat polyclonal Secondary Antibody to Rat IgG has been cross adsorbed against mouse serum proteins and has less than 1% cross reactivity against mouse polyclonal IgG by ELISA testing. I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Sep 28 2010

    Question

    What is the concentration of this antibody? It is lot 168763.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 10 2006

    Answer

    Thank you for your enquiry. The concentration of this antibody is 1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 10 2006

    Question

    I'm afraid I have some fairly basic questions, but I need a quick answer from a reliable source! I'm interested in buying a secondary antibody conjugated to a blue fluorochrome. Firstly, is Cy5.5 a blue fluorochrome as well as Cy5 and if so what is the difference? Also, am I right in thinking that Cy5 and AF 350 are the only blue fluorochromes that can be purchased conjugated to secondary antibodies? I was also wanting to check if these antibodies can be visualised through a DAPI filter on a normal fluorescence microscope and a confocal laser microscope. Providing I am barking up the right tree with these assumptions, I'm looking for an anti-mouse IgG and/or IgM antibody and I'm also considering an anti-rat IgG and an anti-rabbit IgG to use for triple immunolabelling on primary cell cultures (oligodendrocytes) and on paraffin pre-fixed and/or fresh frozen cryostats. Can you give me information on which of these antibodies you supply and particularly any that are good value or supplied in trial size volumes!

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2004

    Answer

    Thanks for your enquiry,unfortunately we do not supply sample sized testers. As far as I am aware both CY5 and CY 5.5 are red. If you log on to our web page and click on secondary antibodies there is a list of all the secondary antibodies we supply along with a fluorophore data table.

    Read More

    Abcam Scientific Support

    Answered on Jun 02 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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