Goat Anti-Rat IgG H&L (Biotin) preadsorbed (ab7096)

Submit a review Q&A (1)References (5)

Key features and details

  • Goat Anti-Rat IgG H&L (Biotin) preadsorbed
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Fr

製品の概要

  • 製品名

    Goat Anti-Rat IgG H&L (Biotin) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rat

  • アプリケーション

    適用あり: ICC/IF, WB, ELISA, Immunomicroscopy, Dot blot, IHC-P, IHC-Frmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details

  • 免疫原

    Full length native Rat IgG (purified).

  • 標識

    Biotin

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 特記事項（標識）

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
Immunomicroscopy
Dot blot
IHC-P
IHC-Fr
  • 追加情報
    Dot: Use at an assay dependant dilution.
    ELISA: 1/20000 - 1/100000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (5)

    ab7096 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7096 は 5 報の論文で使用されています。

    • Anesten F  et al. Preproglucagon neurons in the hindbrain have IL-6 receptor-a and show Ca2+ influx in response to IL-6. Am J Physiol Regul Integr Comp Physiol 311:R115-23 (2016). PubMed: 27097661
    • Fry CS  et al. Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. FASEB J 28:1654-65 (2014). PubMed: 24376025
    • Carramolino L  et al. Platelets play an essential role in separating the blood and lymphatic vasculatures during embryonic angiogenesis. Circ Res 106:1197-201 (2010). PubMed: 20203303
    • Graham JD  et al. DNA replication licensing and progenitor numbers are increased by progesterone in normal human breast. Endocrinology 150:3318-26 (2009). IHC-P . PubMed: 19342456
    • Mote PA  et al. Overlapping and distinct expression of progesterone receptors A and B in mouse uterus and mammary gland during the estrous cycle. Endocrinology 147:5503-12 (2006). PubMed: 16980438

    レビューと Q&A

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    Question

    Looking for CD31, CD54, and CD146 antibodies to work on mouse liver IHC-P.

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    Answer

    Thank you for your call today and for your interest in these antibodies.

    Since we do not have a CD54 antibody that hasalready been tested in IHC-P with mouse samples, I can offer a discount off a future purchase if you'd liketest a CD54 antibodynow, test it in IHC-P, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth one free primary antibody on a future purchase.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test a CD54 antibody in IHC-P. I will then send a discount code.

    2. Purchasethe CD54 antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-P.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the CD54 antibody turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com