Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab7087)

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Key features and details

  • Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed
  • Conjugation: TRITC. Ex: 547nm, Em: 572nm
  • Host species: Goat
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA

製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識

    TRITC. Ex: 547nm, Em: 572nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.

  • 特記事項（標識）

    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • 追加情報
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab7087 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7087 は 1 報の論文で使用されています。

    • Liu Y  et al. PKCd promotes fertilization of mouse embryos in early development via the Cdc25B signaling pathway. Exp Ther Med 18:3281-3290 (2019). PubMed: 31602201

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    Re: the tetra methyl rhodamine isocyanate conjugated to this product. Is the dye used the 5 isomer, 6 isomer, or mixed isomers? ie tetramethylrhodamine-5-isothiocyanate (5-TRITC G isomer), tetramethylrhodamine-6-isothiocyanate (6-TRITC R isomer),or tetramethylrhodamine-5-(and-6)-isothiocyanate (5(6)-TRITC) *mixed isomers*

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    Answer

    Thank you for your recent technical service inquiry. The TRITC used for our conjugations is tetramethlyrodamine-5-(and-6)-isothiocyanate (mixed isomers). Please feel free to contact me for additional assistance regarding this issue.

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    Question

    I would like to ask you the difference between ab27089 and ab4055. I'd also like to know whether your goat anti-rabbit IgG (Rhodamine-conjugated); ab7087 or ab6718, is working well as a secondary antibody for these anti-CD8 polyclonal as well as the difference between ab7087 and ab6718. Thanks in advance.

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    Answer

    Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible secondary Abs are cited at the base of each online data sheet for you to choose from. I would expect any non rabbit species anti rabbit IgG with a suitable conjugation to function. 3)Looking at products Ab7087 and Ab6718 the only obvious difference that I am able to spot is the different concentrations of the product. I will pass this information onto my colleague sin business development since it is possible that we have added a duplicate product in error. Should you have any further questions then please do not hesitate to get back in contact with me.

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