Goat Anti-Rabbit IgG H&L (Texas Red ®) (ab6719)

Submit a review Q&A (1)References (39)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Texas Red ®)
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 20nm Gold 40nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE TRITC Unconjugated Unconjugated

製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (Texas Red ®)
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy, ELISAmore details

  • 免疫原

    Rabbit IgG, whole molecule

  • 標識

    Texas Red ®. Ex: 596nm, Em: 620nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.

  • 特記事項（標識）

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6719の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000 - 1/5000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
特記事項
ICC/IF
1/1000 - 1/5000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (39)

ab6719 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6719 は 39 報の論文で使用されています。

  • Zhao J  et al. Epigenetic activation of FOXF1 confers cancer stem cell properties to cisplatin-resistant non-small cell lung cancer. Int J Oncol 56:1083-1092 (2020). PubMed: 32319573
  • Yu W  et al. LOC101060264 Silencing Suppresses Invasion and Metastasis of Human Colon Cancer. Med Sci Monit 26:e920270 (2020). PubMed: 32077446
  • Mareninova OA  et al. Transgenic expression of GFP-LC3 perturbs autophagy in exocrine pancreas and acute pancreatitis responses in mice. Autophagy 16:2084-2097 (2020). PubMed: 31942816
  • Kim EH  et al. Melatonin-Nrf2 Signaling Activates Peroxisomal Activities in Porcine Cumulus Cell-Oocyte Complexes. Antioxidants (Basel) 9:N/A (2020). PubMed: 33153240
  • Çelik-Uzuner S Enhanced immunological detection of epigenetic modifications of DNA in healthy and cancerous cells by fluorescence microscopy. Microsc Res Tech 82:1962-1972 (2019). PubMed: 31429164
View all Publications for this product

レビューと Q&A

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Question

Hi, Do you know exact position of TR conjugate on antibody structure? I mean does it always bind a specific part of antibody or bind any where on the antibody? As antibody orientation on the gold surface is random I have to know that antibody conjugation to TR is random or not, as well. Thanks Regards

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Answer

Thank you for your enquiry. I can confirm that ab6719 Rabbit IgG secondary antibody has more than 1 molecule of Texas Red per molecule of IgG. During conjugation these molecules are bound randomly to different sites on the IgG molecules. I hope this information is helpful to you. Please do not hesitate to contact us if you have any further questions.

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