Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Key features and details
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
- Conjugation: IRDye® 800CW
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
Related conjugates and formulations
製品の概要
-
製品名
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
IgG 二次抗体 製品一覧 -
製品の詳細
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WB, In-Cell ELISAmore details -
吸着処理血清
Chicken, Human, Mouse, Rat, Sheep more details -
免疫原
Full length protein corresponding to Rabbit IgG.
-
標識
IRDye® 800CW
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab216773の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/25000.
|
|
| In-Cell ELISA | (1) |
1/200 - 1/1200.
|
| 特記事項 |
|---|
|
WB
1/5000 - 1/25000. |
|
In-Cell ELISA
1/200 - 1/1200. |
画像
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)
Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
Lane 2 : Lamin B1 knockout HAP1 nuclear lysate
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Spectrum - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (25)
ab216773 は 25 報の論文で使用されています。
- Pan Y et al. The nature of the modification at position 37 of tRNAPhe correlates with acquired taxol resistance. Nucleic Acids Res 49:38-52 (2021). PubMed: 33290562
- Wei C et al. Overactivated sonic hedgehog signaling aggravates intrauterine adhesion via inhibiting autophagy in endometrial stromal cells. Cell Death Dis 11:755 (2020). PubMed: 32934215
- Luongo TS et al. SLC25A51 is a mammalian mitochondrial NAD+ transporter. Nature 588:174-179 (2020). PubMed: 32906142
- Fu H et al. Brequinar inhibits enterovirus replication by targeting biosynthesis pathway of pyrimidines. Am J Transl Res 12:8247-8255 (2020). PubMed: 33437396
- Wang KJ et al. Piceatannol protects against cerebral ischemia/reperfusion-induced apoptosis and oxidative stress via the Sirt1/FoxO1 signaling pathway. Mol Med Rep 22:5399-5411 (2020). PubMed: 33173979