Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
Key features and details
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
- Conjugation: IRDye® 800CW
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, In-Cell ELISA
製品の概要
-
製品名
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed
IgG 二次抗体 製品一覧 -
製品の詳細
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: WB, In-Cell ELISAmore details -
吸着処理血清
Chicken, Human, Mouse, Rat, Sheep more details -
免疫原
Full length protein corresponding to Rabbit IgG.
-
標識
IRDye® 800CW
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: PBS
Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer. -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Affinity chromatography purified using pooled rabbit IgG covalently linked to agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab216773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/5000 - 1/25000. | |
| In-Cell ELISA | 1/200 - 1/1200. |
画像
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-NF-kB p65 antibody (ab16502) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : NF-kB p65 knockout HAP1 cell lysate (20 µg)
Lane 3 : HeLa cell lysate (20 µg)
Lane 4 : A431 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW))This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16502 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green - ab16502 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1 µg/ml (Anti-Lamin B1 antibody - Nuclear Envelope Marker)
Lane 1 : Wild-type HAP1 nuclear lysate, 10 ug
Lane 2 : Lamin B1 knockout HAP1 nuclear lysate
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilutionThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab16048 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green signal from target - ab16048 observed at 68 kDa. Red signal from loading control - ab10799 ( (loading control to histone H3 at 0.1µg/ml) observed at 18 kDa using as secondary Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776.
-
Other - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)Excitation and emission spectrum of IRDye® 800CW Ex: 776nm, Em: 792nm.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (13)
ab216773 は 13 報の論文で使用されています。
- Tian X et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/ß-catenin signaling. Mol Oncol 14:462-483 (2020). PubMed: 31785055
- Piers TM et al. A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC-derived microglia. FASEB J 34:2436-2450 (2020). PubMed: 31907987
- Tatebe H et al. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge. Nat Commun 11:370 (2020). PubMed: 31953386
- Schukken KM et al. Altering microtubule dynamics is synergistically toxic with spindle assembly checkpoint inhibition. Life Sci Alliance 3:N/A (2020). PubMed: 31980556
- Cao H et al. miR-145-5p Regulates the Proliferation, Migration and Invasion in Cervical Carcinoma by Targeting KLF5. Onco Targets Ther 13:2369-2376 (2020). PubMed: 32256087
