製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細

    Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
  • 由来種

    Goat
  • ターゲット生物種

    Rabbit
  • アプリケーション

    適用あり: WB, In-Cell ELISAmore details
  • 吸着処理血清


    Chicken, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length protein corresponding to Rabbit IgG.

  • 標識

    IRDye® 680RD

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファー

    pH: 7.4
    Preservative: 0.01% Sodium azide
    Constituent: PBS

    Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer.
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    Affinity chromatography purified using pooled IgG covalently linked to agarose.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab216777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/25000.
In-Cell ELISA 1/200 - 1/800.

画像

  • All lanes : Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) at 1/10000 dilution (Anti-GAPDH antibody)

    Lane 1 : Wild-type HAP1 cell lysate (20 µg)
    Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution (Goat anti-Mouse IgG H&L (IRDye® 680RD))


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-GAPDH antibody ab181602 overnight at 4°C. Antibody binding was detected using the Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    Image shows merged signal (red and green). Green - Anti-beta Actin antibody [AC-15] (ab6276)ab6276 observed at 42 kDa. Red - GAPDH loading control, ab181602, observed at 37 kDa.

  • Image taken from In-cell ELISA PhosphoPDH Kit (ab110218), which uses Goat anti-mouse IgG H&L (IRDye® 680RD) preadsorbed ab216777 for detection among its components.
  • Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.

参考文献

ab216777 has not yet been referenced specifically in any publications.

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