Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

製品の概要

  • 製品名
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • 由来種
    Goat
  • ターゲット生物種
    Rabbit
  • 特異性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • アプリケーション
    適用あり: ICC, IHC-P, ELISA, WBmore details
  • 吸着処理血清

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. as primary.
WB 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.

画像

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97080, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with ab87342.

参考文献

This product has been referenced in:
  • Lu Y  et al. Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function. Br J Pharmacol 175:1085-1099 (2018). IHC-P ; Mouse . Read more (PubMed: 29352742) »
  • Cioni JM  et al. Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. Neuron 97:1078-1093.e6 (2018). Read more (PubMed: 29518358) »

See all 22 Publications for this product

レビューと Q&A

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Application
Western blot
This item was applied as a secondary Ab for my WB. 1:10,000 dilution was applied for chemiluminescence reaction afterwards. I also conducted the procedure with secondary Ab probing only (right side of the blot) and the result showed no non-specific binding for this Ab. I am satisfied for this item in my WB assay.
Username

Abcam user community

Verified customer

投稿 Apr 11 2018

Application
Immunohistochemistry (Frozen sections)
Sections were thawed and fixed in cold acetone. Endogenous peroxidase and serum blocking (10% normal horse serum) were performed. Primary antibody was incubated with ab9722, at 5µg/ml overnight at 4°C. An HRP-conjugated secondary (ab97080 at 1/300 dilution) was used for 30min at room temperature. Sections were counterstained with haematoxylin and mounted with DPX.
A negative control was included using an isotype IgG at the same concentration as the primary antibody.
*Staining performed by Maria Wallert.
Username

Ana Maluenda

Verified customer

投稿 Jan 31 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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