製品の概要

  • 製品名
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種
    Goat
  • ターゲット生物種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • 吸着処理血清

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識
    HRP

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 精製度
    Affinity purified
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 特記事項(標識)
    Horseradish Peroxidase (HRP)
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/20000 - 1/80000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

画像

  • Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.

    Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.

    Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

  • ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Chen C  et al. Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells. Oncol Lett 15:4541-4549 (2018). Read more (PubMed: 29541224) »
  • Li Z  et al. Intramedullary central neurocytoma of the thoracic spinal cord: A case report and literature review. Mol Clin Oncol 8:544-548 (2018). Read more (PubMed: 29541463) »
See all 45 Publications for this product

レビューと Q&A

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1-2 of 2 Abreviews

Good abtibody

Excellent
Abreviews
Application
Western blot
I use this antibody mostly in WB. I can get good signals (1/10000 dilution).

Upload image condition:
1st antibody ab108296 1/1000 dilution(5% milk)
2nd antibody This antibody 1/10000 dilution(5% milk)

Abcam user community

Verified customer

投稿 Sep 14 2017

Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-Inos (ab15323) with Goat anti Rabbit HRP (on human artery)
-Deparaffinize
-heat in citrate buffer 20min at 96°C
-H2O2 for 10min at room temperature
-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature
-incubate anti INOS at 1/100 in PBS BSA Overnight at 4°C
-incubate with goat anti rabbit HRP (1/300) for 45min at 37°C
-incubate slides in DAB or other peroxidase substrate
-counterstain with hematoxylin, dehydrate and mount

Abcam user community

Verified customer

投稿 Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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