Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)
Key features and details
- Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Fr
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Full length native Rabbit IgG (purified).
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. -
特記事項(標識)
Horseradish Peroxidase (HRP) -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7090の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/1000 - 1/5000.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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ELISA |
1/20000 - 1/80000.
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Immunomicroscopy |
Use at an assay dependent concentration.
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|
Dot blot |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
|
特記事項 |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ELISA
1/20000 - 1/80000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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Immunohistochemistry - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)Lean QY et al. PLoS One (2015) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis of healthy and colitis mouse colon sections (untreated and treated with enoxaparin) labeling claudin-4 with ab15104 at 1/200. ab7090, anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (HRP) at 1/300 was used as the secondary antibody. ab64238 at 1/50 was used to develop the histological signal.
Antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8.0 or 10 mM citrate buffer, pH 6.0.
Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)This image is courtesy of an anonymous Abreview.
ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (311)
ab7090 は 311 報の論文で使用されています。
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- Hu Y et al. miR-491-3p is Downregulated in Retinoblastoma and Inhibit Tumor Cells Growth and Metastasis by Targeting SNN. Biochem Genet 59:453-474 (2021). PubMed: 33098307
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- Xiao L et al. Radiation exposure triggers the malignancy of non-small cell lung cancer cells through the activation of visfatin/Snail signaling. Oncol Rep 45:1153-1161 (2021). PubMed: 33432364
- Lv M & Liu Q JMJD2C triggers the growth of multiple myeloma cells via activation of ß-catenin. Oncol Rep 45:1162-1170 (2021). PubMed: 33469678