Goat Anti-Rabbit IgG H&L (Glucose Oxidase) (ab136782)
Key features and details
- Goat Anti-Rabbit IgG H&L (Glucose Oxidase)
- Conjugation: Glucose Oxidase
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC, IHC-P, IHC-Fr
Related conjugates and formulations
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APC
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Biotin
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FITC
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HRP
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PE
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TRITC
Unconjugated
Unconjugated
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Glucose Oxidase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: ELISA, WB, ICC, IHC-P, IHC-Frmore details -
吸着処理血清
Human more details -
免疫原
Highly purified whole rabbit immunoglobulin.
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標識
Glucose Oxidase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituents: 50% Glycerol, 50% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab136782 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab136782の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/100 - 1/1000. Substrate:
10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm.
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|
WB |
1/400.
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|
ICC |
1/10 - 1/100. Substrate:
15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness.
|
|
IHC-P |
1/10 - 1/100.
|
|
IHC-Fr |
1/10 - 1/100.
|
特記事項 |
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ELISA
1/100 - 1/1000. Substrate: 10mg/ml D(+)glucose, 0.1mg/ml grade II (Rz=0.6) peroxidase, 0.35mg/ml OPD in 0.1M pH5 citrate-phosphate buffer. Incubation 30 minutes at 37°C. Stop the reaction with V/2 volume of 2N H2SO4. Reading at 492 nm. |
WB
1/400. |
ICC
1/10 - 1/100. Substrate: 15mg/ml D(+)glucose, 0.5mg/ml blue thiazolyl, 0.35mg/ml phenazine methosulfonate to be dissolved in that order in a 0.1M pH6.8 phosphate buffer. Incubation 20 to 30 minutes in darkness. |
IHC-P
1/10 - 1/100. |
IHC-Fr
1/10 - 1/100. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab136782 は論文での使用が確認できていません。