Goat Anti-Rabbit IgG H&L (FITC) (ab97050)
Key features and details
- Goat Anti-Rabbit IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (FITC)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details -
免疫原
Full length protein. Rabbit IgG (containing the usual heavy and light chain)
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC. -
特記事項（標識）
F/P ratio is 4.85 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97050の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/500.
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| ICC/IF |
1/50 - 1/500.
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| Flow Cyt |
1/50 - 1/200.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/500. |
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ICC/IF
1/50 - 1/500. |
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Flow Cyt
1/50 - 1/200. |
画像
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)Ren Y et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014).
Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed Triton X-100 permeabilized Goat skeletal muscle-derived satellite cells stained for NSE using ab53025.
After washing in PBS, the cells were incubated with Goat Anti-Rabbit IgG H&L (FITC) (ab97050) (Green).
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Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)This image is courtesy of an anonymous Abreview.ab84235 staining melanoma inhibitory activity in a human melanoma cell line by Flow Cytometry. Cells were harvested with EDTA and washed in PBS. Cells were pemeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. A FITC-conjugated goat anti-rabbit IgG H&L (ab97050) (1/100) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibodies:
Mouse anti-Ki67, 1:100
Rabbit anti-laminin alpha 1 (basement marker)
Secondary antibodies:
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100 -
Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.
This image is courtesy of an anonymous Abreview
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Immunohistochemistry (Frozen sections) - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4° overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
Nuclei were counterstained stained with DRAQ7™ (ab109202), 1:1000 -
Flow Cytometry - Goat Anti-Rabbit IgG H&L (FITC) (ab97050)The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer. We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C. The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.
This image is courtesy of an anonymous Abreview
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab97050 は 36 報の論文で使用されています。
- Iwamoto K et al. Autologous transplantation of multilayered fibroblast sheets prevents postoperative pancreatic fistula by regulating fibrosis and angiogenesis. Am J Transl Res 13:1257-1268 (2021). PubMed: 33841654
- Thompson ER et al. Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation. Am J Transplant 21:1402-1414 (2021). PubMed: 32506663
- Kappes L et al. Ambrisentan, an endothelin receptor type A-selective antagonist, inhibits cancer cell migration, invasion, and metastasis. Sci Rep 10:15931 (2020). PubMed: 32985601
- Yotsumoto T et al. NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer. Oncotarget 11:3590-3600 (2020). PubMed: 33062195
- Pelot NA et al. Quantified Morphology of the Cervical and Subdiaphragmatic Vagus Nerves of Human, Pig, and Rat. Front Neurosci 14:601479 (2020). PubMed: 33250710