Goat Anti-Rabbit IgG H&L (DyLight® 594) (ab96885)

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in many cases, regrettably we do not have access to all the articles mentioning our products, or subscriptions to all the journals that publish them. I'm sorry there are none to provide on this occasion for ab93350 and ab96885.

Should you have any further questions, please do not hesitate to contact us.

Thank you for contacting Abcam regarding ab96885.


The conjugate should be relatively stable during storage in the dark. In our hands, we've read the same stained tissue every min for 10 min, without noticeable loss in intensity. Similarly, we stored the covered tissue at RT for more than a month and found no significant difference in fluorescence intensity.


For your specific situation, the fluorescence intensity should not be noticeably different after 72 hours. However, if you are performing tight quantitative measurements, it is always better to make preliminary tests, just to make sure.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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I can confirm the Safety Data Sheets can be found on the online datasheets. Near the top right hand side of the datasheet, there are two 'buttons', one for Datasheet PDF and the one underneath says SDS. Click on the SDS button to link to the document.

I hope this will be helpful. If you have any difficulties finding these, or if you have any further questions, please do not hesitate to contact me.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from abab96885 Goat anti-Rabbit IgG H&L (DyLight® 594) secondary antibody. I would also appreciate if you can confirm some further details:

1. What kind of filter or lamp are you using? Unfortunately, DyLight® 488and DyLight® 594 do overlap: a little bit in the DyLight® 488 spectrum, but more important in the DyLight® 594 wavelengths, which corresponce with your findings. Check with the manufacturer of your microscope if their filters are suitable for those dyes.

2. In order to check if the ab96885 is damaged I would suggest to perform an ICC experimentwith only one primary- secondaryreaction. This way you canexclude the annoying "bleed-over".


Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for your inquiry.


This secondary antibody is Dylight 594 conjugated, which is why we recommend storing it at 4C. Freezing this antibody can affect the fluorophore. You can try it with a rabbit primary antibody to see if it can be detected and the fluorophore is still viable.


At this point it is difficult to say if it will work or not without testing it because we haven't stored it frozen in our labs. I am sorry that this information could not be more helpful, but please contact us with any other questions.

My colleague forwarded your message about testing an Abcam IGF2 antibody to me.

As he mentioned, if western blotting and IHC are listed as applications on the datasheet, and rat and mouse are listed, then the antibody will be guaranteed for those applications and species and a replacement or credit or refund is available in the event it fails. If not, it is not guaranteed.

For untested applications or species, we do not have samples, but we offer the testing discount he mentioned.

The procedure is as follows: I first issue a discount code to you. Then, your lab purchases the antibody, tests it, and sends us your results in the form of a review (www.abcam.com/abreviews has some information about this). Once we have your review, your testing discount code becomes active, regardless of whether the results are positive or negative.

The discount is a credit that can be applied to a subsequent order, good for one free primary antibody of your choice. I will be happy to issue you a code before you purchase. I just need the antibody catalogue number, and I will send you a code, which you include in the notes section of the review form.

For some examples of customer reviews, please see the datasheet for anti-IGF2 ab9574:

Click here (or use the following: https://www.abcam.com/IGF2-antibody-ab9574.html).

Regarding a control peptide, this is available for some antibodies, but not all. If so, it will be a separate item for purchase.

I look forward to your reply.

Thank you for your response.

We do not sell Alexa-conjugated secondary antibodies. However, we have launched a new family of DyLight® fluorochrome- labelled secondaries which exhibit higher fluorescence intensity, photostability and water solubility and remain fluorescent from pH 4 to pH 9.

Additionally, the water solubility of the DyLight® fluorochromes allows a high dye-to-antibody ratio to be achieved without causing precipitation of conjugates. You customer can find some further useful details on DyLight® secondary antibodies at our home site:

https://www.abcam.com/index.html?pageconfig=resource&rid=12934

I can confirm that both ab96885 and ab96889 have been tested for Flow cytometry and ICC/IF. However, please check with your customer the compatibility with the primary antibody(ies) before making the final purchase decision.

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® fluorochromes. This family of dyes is comparable to AlexaFluor dyes regarding high fluorescence intensity, photostability and water solubility. Both the absorption and emission properties of the DyLight® fluorochomes are compatible with the excitation and detection wavelengths of common fluorescence instrumentation (https://www.abcam.com/DyLight). Please be reassured that all our products are covered by our Abpromise: We guarantee our products work in the tested species and applications as stated on the datasheet, and we will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. Thus, please see a selection of our options: 1+2) Unfortunately, we currently do not have any phalloidin protein or antibody against phalloidin available. 3) ab96873, ab96881: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-594-ab96873.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-594-preadsorbed-ab96881.html). 4) ab96871, ab96871, ab97022: Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html). Click here (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-FITC-ab97022.html). 5) ab102883, ab102884, ab102885 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html). Click here (or use the following: https://www.abcam.com/FITC-Conjugation-Kit-Lightning-Linkreg-ab102884.html). Click here (or use the following: https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-10g-FITC-ab102885.html). 6) ab102923, ab1029234, ab102925 (please see https://www.abcam.com/EasyLink or attachment for more details): Click here (or use the following: https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-1-x-1mg-TR-ab102923.html). Click here (or use the following: https://www.abcam.com/Texas-Redreg-Conjugation-Kit-ab102924.html). Click here (or use the following: https://www.abcam.com/EasyLink-Texas-Red-Conjugation-Kit-3-x-10g-TR-ab102925.html). 7) ab97050, ab96883, ab96899: Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-FITC-ab97050.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-ab96883.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html). 8) ab96885, ab96897: Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-594-ab96885.html). Click here (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-594-preadsorbed-ab96897.html). We also have the secondary antibodies in different formats (pre-absorbed, Fab fragments etc) which might be more suitable for certain applications. I will be happy to assist you in finding the most appropriate product on request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.