Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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アプリケーション
適用あり: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
吸着処理血清
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
標識
Cy5 ®. Ex: 650nm, Em: 667nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/50 - 1/500.
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Flow Cyt |
1/100 - 1/1000.
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IHC-P |
1/50 - 1/500.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/100 - 1/1000. |
IHC-P
1/50 - 1/500. |
画像
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Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells.
Rab7 was detected with an Rabbit anti-Rab7 antibody, followed by ab97077 at 1/500 dilution in ICC/IF analysis of HeLa cells.
(From Figure S1 of Humphries et al)
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Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Preparation:- Fix in 3% PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:100
Nuclei were counterstained with SYTO 16 -
ab97077 (1/200) detecting ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (34)
ab97077 は 34 報の論文で使用されています。
- Yoshida Y et al. Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue. iScience 25:104268 (2022). PubMed: 35521515
- Hayashi Y et al. Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity. iScience 25:104547 (2022). PubMed: 35754738
- Yoshida Y et al. Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism. Sci Rep 12:14883 (2022). PubMed: 36050466
- Schmiderer L et al. Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import. Sci Rep 12:18687 (2022). PubMed: 36333335
- Bouchard G et al. Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge. Cancer Res 82:648-664 (2022). PubMed: 34853070