Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed (ab6941)

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Key features and details

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3.5 ®), pre-adsorbed
  • Conjugation: Cy3.5 ®. Ex: 581nm, Em: 596nm
  • Host species: Goat
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 20nm Gold 40nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (Cy3.5 ®) preadsorbed
    IgG 二次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3.5 ®), pre-adsorbed

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識

    Cy3.5 ®. Ex: 581nm, Em: 596nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above

  • 特記事項（標識）

    Cy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1286 daltons) Absorption Wavelength: 581 nm Emission Wavelength: 596 nm Fluorochrome/Protein Ratio: 9.3 moles Cy3.5 per mole of Goat IgG

  • ポリ/モノ

    ポリクローナル

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6941の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
特記事項
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (4)

ab6941 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6941 は 4 報の論文で使用されています。

  • Mohapatra S  et al. Power of an Organic Electron Acceptor in Modulation of Intracellular Mitochondrial Reactive Oxygen Species: Inducing JNK- and Caspase-Dependent Apoptosis of Cancer Cells. ACS Omega 6:7815-7828 (2021). PubMed: 33778293
  • Weingarten G  et al. Insulin receptor plays a central role in skin carcinogenesis by regulating cytoskeleton assembly. FASEB J 33:2241-2251 (2019). PubMed: 30332298
  • Ramalho AF  et al. Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface-the protective role for BDNF. J Neuroinflammation 15:10 (2018). IHC-P ; Mouse . PubMed: 29316939
  • Fioravante M  et al. Inhibition of hypothalamic leukemia inhibitory factor exacerbates diet-induced obesity phenotype. J Neuroinflammation 14:178 (2017). PubMed: 28865476

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