Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Full length native Rabbit IgG (purified).
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標識
Cy3 ®. Ex: 552nm, Em: 565nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. -
特記事項(標識)
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/1000 - 1/5000.
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IHC-Fr | (1) |
Use at an assay dependent dilution.
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IHC-P |
1/100. PubMed: 17045908
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ICC |
Use at an assay dependent dilution.
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Flow Cyt |
1/500 - 1/2500.
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ELISA |
1/10000 - 1/50000.
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特記事項 |
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ICC/IF
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent dilution. |
IHC-P
1/100. PubMed: 17045908 |
ICC
Use at an assay dependent dilution. |
Flow Cyt
1/500 - 1/2500. |
ELISA
1/10000 - 1/50000. |
画像
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.
IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).
Photomicrographs are representative of four independent samples.
Cell nuclei were counterstained with DAPI (blue).
Original magnification: 200x.
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Examples of the different antibody stainings for functional analyses.
Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).
Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).
Original magnifications are indicated in the images.
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Lane M: 3 µL Molecular ladder.
Lane 1: Rabbit IgG whole molecule (p/n 011-0102).
Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).
Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).
Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).
Lane 5: Normal Rabbit Serum (p/n B309).
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.
Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
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Lane 1: Rabbit IgG.
Load: 50 ng per lane.
Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (147)
ab6939 は 147 報の論文で使用されています。
- Lu N et al. miRNA-490-3p promotes the metastatic progression of invasive ductal carcinoma. Oncol Rep 45:706-716 (2021). PubMed: 33416185
- Zhu J et al. MicroRNA-140-5p regulates the proliferation, apoptosis and inflammation of RA FLSs by repressing STAT3. Exp Ther Med 21:171 (2021). PubMed: 33456538
- Xie QQ et al. Nogo-66 promotes ß-amyloid protein secretion via NgR/ROCK-dependent BACE1 activation. Mol Med Rep 23:N/A (2021). PubMed: 33495810
- Wang J et al. SCARA5 suppresses the proliferation and migration, and promotes the apoptosis of human retinoblastoma cells by inhibiting the PI3K/AKT pathway. Mol Med Rep 23:N/A (2021). PubMed: 33495818
- Miao J et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963