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Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

Reviews (3)Q&A (3)References (147)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
  • Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
  • Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
  • Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Full length native Rabbit IgG (purified).

  • 標識

    Cy3 ®. Ex: 552nm, Em: 565nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.

  • 特記事項（標識）

    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6939の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/1000 - 1/5000.
IHC-Fr (1)
Use at an assay dependent dilution.
IHC-P
1/100. PubMed: 17045908
ICC
Use at an assay dependent dilution.
Flow Cyt
1/500 - 1/2500.
ELISA
1/10000 - 1/50000.
特記事項
ICC/IF
1/1000 - 1/5000.
IHC-Fr
Use at an assay dependent dilution.
IHC-P
1/100. PubMed: 17045908
ICC
Use at an assay dependent dilution.
Flow Cyt
1/500 - 1/2500.
ELISA
1/10000 - 1/50000.

画像

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Bockor et al PLoS One. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954. eCollection 2014. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Histone modifications associated with NOR-1 and NOR-2 loci in root tip cycling cells of Q.robur.

    IFF detection of H3K9ac (A), H3K4me3 (B), H3K9me1 (C) and H3K27me2 (D). Green signals correspond to immunofluorescence of histone antibodies and red signals represent 18S rDNA FISH signals. Letter “n” marks the nucleoli. Scale bar is 5 µm.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Islam et al PLoS One. 2015 Jun 8;10(6):e0128306. doi: 10.1371/journal.pone.0128306. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Photomicrographs showing immunostaining of (Panel A) ABCG2 (red), (Panel B) Bmi-1 (green), (Panel C) C/EBPδ (red), (Panel D) PCNA (red), (Panel E) CK18 (green), and (Panel F) cleaved caspase-3 (red) in HOK (human oral keratinocytes) cells cultured for five days without subsequent storage (control).

    Photomicrographs are representative of four independent samples.

    Cell nuclei were counterstained with DAPI (blue).

    Original magnification: 200x.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)Buchholz et al PLoS One. 2015 Apr 7;10(4):e0122946. doi: 10.1371/journal.pone.0122946. eCollection 2015. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Examples of the different antibody stainings for functional analyses.

    Left Panel: Ki-67 staining (Cy-3, red), HEK-293 transfected with NKX2-5-YFP (green).

    Right Panel: Cyclin B staining (Cy-3, red), PANC-1 transfected with PRKCZ-YFP (green).

    Original magnifications are indicated in the images.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

    ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

  • Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

    Lane M: 3 µL Molecular ladder.

    Lane 1: Rabbit IgG whole molecule (p/n 011-0102).

    Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105).

    Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103).

    Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107).

    Lane 5: Normal Rabbit Serum (p/n B309).

    All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.

    Primary Antibody: ab6939 used at 1/1000 for 60 min at RT.

    Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody used at 1/40000 in blocking buffer for 30 min at RT.

    Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.

     

    Rabbit F(c) migrates slightly higher.

  • Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)
    Western blot - Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

    Lane 1: Rabbit IgG.

    Load: 50 ng per lane.

    Secondary antibody: ab6939 at 1/1000 for 60 min at RT. Blocked for 30 min at RT.

    Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Rabbit IgG.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (147)

ab6939 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6939 は 147 報の論文で使用されています。

  • Lu N  et al. miRNA-490-3p promotes the metastatic progression of invasive ductal carcinoma. Oncol Rep 45:706-716 (2021). PubMed: 33416185
  • Zhu J  et al. MicroRNA-140-5p regulates the proliferation, apoptosis and inflammation of RA FLSs by repressing STAT3. Exp Ther Med 21:171 (2021). PubMed: 33456538
  • Xie QQ  et al. Nogo-66 promotes ß-amyloid protein secretion via NgR/ROCK-dependent BACE1 activation. Mol Med Rep 23:N/A (2021). PubMed: 33495810
  • Wang J  et al. SCARA5 suppresses the proliferation and migration, and promotes the apoptosis of human retinoblastoma cells by inhibiting the PI3K/AKT pathway. Mol Med Rep 23:N/A (2021). PubMed: 33495818
  • Miao J  et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Westerns with Color

 Good
Abreviews
abreview image
Application
Western blot
I recently used this secondary while trying to get a multiplex western to work. I was able to detect bands with this secondary using a G.E. Typhoon FLA 7000. I was able to detect bands with a minimal about of crossover. My only problem was that I had to use the antibody at 1:1000, which is rather concentrated for a fluorescent western.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 01 2022

Immunofluorescene staining of monocytes

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Excellent antibody
Cells: Monocytes
Dilution 1:1000
Primary antibody: Rabbit anti-CD14
Counterstained with hematoxylin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 09 2021

Great secondary antibody for frozen tissue section

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
This antibody was used to study the CD44-expression in human melanoma by indirect immunfluorescence.
The anti rabbit antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results.
Concentration 1:500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 28 2021

Question

Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early response requested.



Sincere regards,

Read More
Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam1012-ChIP-Grade-ab1012.html).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-mAbcam12209-ChIP-Grade-ab12209.html).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html (or use the following: https://www.abcam.com/Histone-H3-tri-methyl-K27-antibody-mAbcam-6002-ChIP-Grade-ab6002.html).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html (or use the following: https://www.abcam.com/DDDDK-tag-antibody-Equivalent-to-FLAG-antibodies-from-Sigma-ab124462.html).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-Cy3--preadsorbed-ab6939.html).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html (or use the following: https://www.abcam.com/Goat-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96899.html).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-DyLight-488-preadsorbed-ab96919.html).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-preadsorbed-ab96879.html).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-HL-DyLight-488-ab96871.html).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG-Fc-HRP-ab97265.html).

ab7481 (Goat Serum)

https://www.abcam.com/Normal-Goat-Serum-ab7481.html (or use the following: https://www.abcam.com/Normal-Goat-Serum-ab7481.html).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html (or use the following: https://www.abcam.com/Normal-Rabbit-Serum-Sterile-ab7487.html).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html (or use the following: https://www.abcam.com/Herring-sperm-ssDNA-ab46666.html).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html (or use the following: https://www.abcam.com/Natural-cow-Ribonuclease-A-protein-ab52579.html).

ab73430 (DNase I protein (Active))

https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html (or use the following: https://www.abcam.com/Recombinant-human-DNase-I-protein-Active-ab73430.html).

ab64220 (Proteinase K)

https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html (or use the following: https://www.abcam.com/Proteinase-K-Antigen-Retrieval-Solution-ab64220.html).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html (or use the following: https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html (or use the following: https://www.abcam.com/Prestained-Protein-Ladder-Broad-molecular-weight-10-245-kDa-ab116028.html).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question

Dear Tech support

Attached is a customer complaint form.
Please advise.
Regards,

Read More
Answer

Thank you very much for the reply.

It is clear from the images provided that the customer has observed some background with the secondary antibody. In order to reduce this, I would advise reducing the concentration which is currently being used. We would recommend initially trying 1/1000-1/5000 for the experiment the customer is currently trying.

I hope this information has been of help. If the customer continues to have problems following this advice please do let us know and we will endeavor to help further.

I hope you ave a nice weekend.

Read More

Question

Dear Tech support

Attached is a customer complaint form.
Please advise.
Regards,

Read More
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to understand what may be contributing to the problems you have encountered, could you please provide me with a few additional details:

1. Which primary antibody was used in the staining? Catalogue number and supplier?

2. Which other secondary antibody was used? Catalogue number and supplier?

3. Would it be possible to provide some images of the staining produced with the ab6939 and the alternative antibody you used in order to understand the differences observed?

4. Could you please provide me with the order number on which ab6939 was purchased?

I look forward to receiving your reply.

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