Goat Anti-Rabbit IgG H&L (beta-galactosidase) (ab136774)
Key features and details
- Goat Anti-Rabbit IgG H&L (beta-galactosidase)
- Conjugation: beta-galactosidase
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC, IHC-P, IHC-Fr
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HRP
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PE
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Unconjugated
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製品の概要
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製品名
Goat Anti-Rabbit IgG H&L (beta-galactosidase)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
ab136774 does not cross react with Human IgG, A, M.
Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems. -
アプリケーション
適用あり: ELISA, WB, ICC, IHC-P, IHC-Frmore details -
吸着処理血清
Human more details -
免疫原
Highly purified whole immunoglobulin from rabbit.
-
標識
beta-galactosidase
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab136774 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab136774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/100 - 1/1000.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
1/100 - 1/1000. |
|
WB
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab136774 は論文での使用が確認できていません。
