製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)
    IgG 二次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 647)
  • 由来種

    Goat
  • ターゲット生物種

    Rabbit
  • 特異性

    This antibody is specific to Rabbit IgG
  • アプリケーション

    適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab150079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/2000.

画像

  • ICC/IF image of ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 2µg/ml) overnight at +4°C. The secondary antibody ab150079 (shown in red) was used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (ab182016).

  • Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182016).

参考文献

This product has been referenced in:

  • Liang C  et al. Y-box binding protein-1 promotes tumorigenesis and progression via the epidermal growth factor receptor/AKT pathway in spinal chordoma. Cancer Sci 110:166-179 (2019). Read more (PubMed: 30426615) »
  • He Y  et al. Dual-functional fluorescent molecular rotor for endoplasmic reticulum microviscosity imaging during reticulophagy. Chem Commun (Camb) 55:2453-2456 (2019). Read more (PubMed: 30734806) »
See all 61 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
E10.5 mouse embryo section. Samples were fixed with PFA and stained before antigen retrieval with sodium citrate.

Primary: FOXA2 ab40874 (1:200).
Secondary: Alexa Fluor 647 ab150079 (1:1000).

Abcam user community

Verified customer

投稿 Jan 27 2014

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- Have you used correct filters? The ab150079 absorption maxima is 650 nm while for FITC (ab98692) it is 495.
- Were the cells permeabilized?
- Is the sequence of insert protein is interpreted. It may well be that the insert sequence is missing which ab128251 binds.
The antibodies ab128251 and a84815 are raised against different part of the immunogen.
- Have you tried optimizing the results by incubating the antibody overnight or by increasing the concentration?

In order for checking the quality of ab150079 you may put a small drop of ab on slide and then seeing it under microscope. If it emits red colour then conjugate is fine and the problem may be with other steps. If it's not then problem is with secondary antibody.

It will be very helpful if you could provide the protcol followed step by step.

Please note that flow cytometry is not a tested application for ab128251 so the Abreview guarantee will not be valid. However if this antibody fails in ELISA, WB and ICC/IF then guarantee will apply as normal product.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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