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  1. Link

    goat-rabbit-igg-hl-alexa-fluor-555-ab150078.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (99)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

Key features and details

  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt

Conjugates logo Related conjugates and formulations

Agarose Alexa Fluor® 405 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 680 Alexa Fluor® 790 Alkaline Phosphatase APC beta-galactosidase Biotin Cy2 ® Cy3 ® Cy3.5 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Glucose Oxidase Gold 12nm Gold 6nm HRP HRP polymer IRDye® 680RD IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated Unconjugated Unconjugated

こちらの製品もご検討ください

二次抗体
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) (ab150114)
一次抗体
Product image
Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

関連製品

製品の概要

  • 製品名

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555)
    IgG 二次抗体 製品一覧
  • 由来種

    Goat
  • ターゲット生物種

    Rabbit
  • アプリケーション

    適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 555

関連製品

  • Alternative Versions

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175471)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175652)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175773)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781)
    • Goat Anti-Rabbit IgG H&L (ab182016)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab150078の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF (1)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration. Use at an assay dependent dilution
IHC-P (1)
Use at an assay dependent concentration. Use at an assay dependent dilution
Flow Cyt
1/2000.
特記事項
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration. Use at an assay dependent dilution
IHC-P
Use at an assay dependent concentration. Use at an assay dependent dilution
Flow Cyt
1/2000.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)This image is courtesy of an abreview by Zachary Yu-Ching Lin

    This data was done using a primary antibody to Calmegin (EPR11832) ab171971. The primary antibody concentration was 1: 100 and the secondary antibody concentration was 1:500.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150078 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

    Cross-reactivity of the polyclonal secondary antibody ab182016 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182016 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182016 showed a cross-reactivity of 5-7% towards Human IgG and below 2% towards Mouse IgG, Rat IgG and Chicken IgY.

    This data was developed using the unconjugated antibody (ab182016).

  • ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    ELISA - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

    Cross-reactivity of Goat anti-Rabbit IgG H&L (ab182016) and Goat anti-Rabbit IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182016).

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

    HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150078 Alexa Fluor® 555 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (99)

ab150078 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab150078 は 99 報の論文で使用されています。

  • Cong X  et al. Silencing GnT-V reduces oxaliplatin chemosensitivity in human colorectal cancer cells through N-glycan alteration of organic cation transporter member 2. Exp Ther Med 21:128 (2021). PubMed: 33376510
  • Sun Y  et al. Homoharringtonine inhibits fibroblasts proliferation, extracellular matrix production and reduces surgery-induced knee arthrofibrosis via PI3K/AKT/mTOR pathway-mediated apoptosis. J Orthop Surg Res 16:9 (2021). PubMed: 33407698
  • Park S  et al. Nogo-A regulates myogenesis via interacting with Filamin-C. Cell Death Discov 7:1 (2021). PubMed: 33414425
  • Liang D  et al. Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nat Commun 12:287 (2021). PubMed: 33436583
  • Zhao D  et al. The inhibitive effects of proteasome inhibitor MG-132 on pterygium fibroblasts in vitro and the potential key regulators involved. Life Sci 270:119088 (2021). PubMed: 33482188
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

E-cadherin staining on rabbit liver tissue

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Application: Immunocytochemistry/ Immunofluorescence
Tissue was fixed in 10% neutral buffered formalin.
Permeabilization: Triton X-100
Primary antibody: Rabbit polyclonal anti-ecadherin
Blocking: 5% BSA in PBS for 20 min
Counterstained with DAPI

DR. Egi Kardia

Verified customer

投稿 Apr 29 2019

Works perfectly with rabbit antibodies.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This data was done using a primary antibody to Calmegin (EPR11832) ab171971. The primary antibody concentration was 1: 100 and the secondary antibody concentration was 1:500.

DR. 煜欽 林

Verified customer

投稿 Oct 04 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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