Goat Anti-Rabbit IgG H&L (ab2892)
Key features and details
- Goat Anti-Rabbit IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, IHC-Fr, ICC
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Rabbit IgG H&L
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
This antibody cocktail reacts with heavy and light chains on rabbit IgG and mouse IgG (reacts with all IgG subtypes). -
アプリケーション
適用あり: IHC-P, IHC-Fr, ICCmore details
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.04% Proclin 300, 0.5% Proclin 950, 99% Water -
Concentration information loading...
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特記事項(標識)
HRP or AP conjugated to a polymer backbone, which is collectively conjugated to the antibody. The end result is two antibody subsets: 1) Multiple HRP molecules linked to goat anti-rabbit antibodies. 2) Multiple AP molecules linked to goat anti-mouse antibodies. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
This product is specially designed for a rapid double stain procedure. The overall staining procedure can be done in 4 steps and in less than two hours. Avidin-biotin blocking procedures are not necessary when using this product. This new and innovative AP and HRP polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP or HRP conjugated secondary antibodies.
This product is provided in a pre-diluted, ready-to-use format. Procedure (adapt in-house protocols to accommodate): 1) A primary antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal/polyclonal antibody must be applied for the standard duration. Rinse in TBS. (DO NOT USE PBS). 2) Apply ab2892 for 20 - 30 minutes then rinse in TBS (DO NOT USE PBS). 3) Chromogen 1: Develop AP using suitable chromogen. Rinse in TBS. 4) Chromogen 2: Develop HRP using suitable chromogen. Rinse in TBS. Equally suited to both manual and automated immunostaining proceedures. This product has not been tested in any application requiring further dilution of the antibody and therefore it is not recommended for use in such applications.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/1.
|
|
IHC-Fr |
1/1.
|
|
ICC |
1/1.
|
特記事項 |
---|
IHC-P
1/1. |
IHC-Fr
1/1. |
ICC
1/1. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab2892 は論文での使用が確認できていません。