Goat Anti-Rabbit IgG Fc (HRP) (ab97200)
Key features and details
- Goat Anti-Rabbit IgG Fc (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
製品の概要
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製品名
Goat Anti-Rabbit IgG Fc (HRP)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rabbit -
特異性
By immunoelectrophoresis, this antibody reacts specifically with Rabbit IgG-Fc Fragment. This antibody may cross react with IgG from other species.
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アプリケーション
適用あり: ICC, IHC-P, ELISA, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000. (Primary ELISA)
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WB |
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)
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特記事項 |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. (Primary ELISA) |
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000) |
画像
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IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (66)
ab97200 は 66 報の論文で使用されています。
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- Liskova V et al. Cystathionine β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells. Front Oncol 13:1178021 (2023). PubMed: 37483514
- Liskova V et al. Camptothecin, triptolide, and apoptosis inducer kit have differential effects on mitochondria in colorectal carcinoma cells. FEBS Open Bio 12:913-924 (2022). PubMed: 35318813
- Cai G et al. SIRT4 functions as a tumor suppressor during prostate cancer by inducing apoptosis and inhibiting glutamine metabolism. Sci Rep 12:12208 (2022). PubMed: 35842463
- Cabral SE et al. Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes. iScience 25:104811 (2022). PubMed: 35982794