Goat Anti-Mouse IgM mu chain (HRP) (ab97230)
Key features and details
- Goat Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, WB, ICC, IHC-P
Related conjugates and formulations
製品の概要
-
製品名
Goat Anti-Mouse IgM mu chain (HRP)
IgM 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. -
アプリケーション
適用あり: ELISA, WB, ICC, IHC-Pmore details -
標識
HRP
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97230の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
1/10000 - 1/100000. (Primary ELISA)
|
|
WB |
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000)
|
|
ICC |
Use at an assay dependent dilution.
|
|
IHC-P |
1/200 - 1/2000.
|
特記事項 |
---|
ELISA
1/10000 - 1/100000. (Primary ELISA) |
WB
1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000) |
ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
画像
-
IHC image of Hsp70 staining in human lung formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab5442, 1/2000 dilution overnight at +4°C. An HRP-conjugated secondary (ab97230, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (48)
ab97230 は 48 報の論文で使用されています。
- Stoler-Barak L et al. B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation. Nat Commun 14:1462 (2023). PubMed: 36927854
- Affonso de Oliveira JF et al. COVID-19 vaccines based on viral nanoparticles displaying a conserved B-cell epitope show potent immunogenicity and a long-lasting antibody response. Front Microbiol 14:1117494 (2023). PubMed: 37152732
- Silva-Cayetano A et al. Spatial dysregulation of T follicular helper cells impairs vaccine responses in aging. Nat Immunol 24:1124-1137 (2023). PubMed: 37217705
- Liu Y et al. O-GlcNAcylation promotes topoisomerase IIα catalytic activity in breast cancer chemoresistance. EMBO Rep 24:e56458 (2023). PubMed: 37249035
- Liu Y et al. FOXA1 O-GlcNAcylation-mediated transcriptional switch governs metastasis capacity in breast cancer. Sci Adv 9:eadg7112 (2023). PubMed: 37595040