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Goat Anti-Mouse IgG3 heavy chain (HRP) (ab97260)

Submit a review Q&A (1)References (10)

Key features and details

  • Goat Anti-Mouse IgG3 heavy chain (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC, IHC-P, ELISA, WB

製品の概要

  • 製品名

    Goat Anti-Mouse IgG3 heavy chain (HRP)
    IgG3 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG3. Cross reactivity with other immunoglobulins is less than 2%.

  • アプリケーション

    適用あり: ICC, IHC-P, ELISA, WBmore details

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • 特記事項（標識）

    Molar enzyme/ antibody protein ratio is 4:1

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97260の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (as Primary)
WB
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000
特記事項
ICC
Use at an assay dependent dilution.
IHC-P
1/200 - 1/2000.
ELISA
1/10000 - 1/100000. (as Primary)
WB
1/2000 - 1/25000. Colorimetric 1/2000 - 1/20000; Chemiluminescent 1/5000 - 1/25000

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (10)

ab97260 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab97260 は 10 報の論文で使用されています。

  • Lainšcek D  et al. A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response. Vaccines (Basel) 9:N/A (2021). PubMed: 33925446
  • Evtushenko EA  et al. Plant virus particles with various shapes as potential adjuvants. Sci Rep 10:10365 (2020). PubMed: 32587281
  • Gupta A  et al. A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLoS Pathog 16:e1009030 (2020). PubMed: 33175909
  • Wang X  et al. Liposomes with cyclic RGD peptide motif triggers acute immune response in mice. J Control Release 293:201-214 (2019). PubMed: 30527753
  • Bellone S  et al. Exceptional Response to Pembrolizumab in a Metastatic, Chemotherapy/Radiation-Resistant Ovarian Cancer Patient Harboring a PD-L1-Genetic Rearrangement. Clin Cancer Res 24:3282-3291 (2018). PubMed: 29351920
  • Lantos I  et al. Chlamydia pneumoniae Infection Exacerbates Atherosclerosis in ApoB100only/LDLR-/- Mouse Strain. Biomed Res Int 2018:8325915 (2018). PubMed: 29770337
  • Pelekanou V  et al. CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival differently in ER-positive and -negative cancers. Breast Cancer Res 20:154 (2018). PubMed: 30558648
  • Liu SA  et al. Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice. J Virol 91:N/A (2017). ELISA . PubMed: 28404844
  • Zhu M  et al. Mechanism of protective immunity by vaccination with recombinant Echinococcus granulosus glutathione S-transferase (Chinese strain) in mice. Exp Ther Med 10:1127-1132 (2015). PubMed: 26622451
  • Kurosaki T  et al. Development of effective cancer vaccine using targeting system of antigen protein to APCs. Pharm Res 29:483-9 (2012). ELISA ; Mouse . PubMed: 21887598

レビューと Q&A

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レビューを送る 質問を送る

Question

I have some specifictechnical questions regarding your secondary antibodies:

1) What level of cross-reactivity is there between thefollowing four antibodies(ie how specific for their IgG subclass target):

mouse mAb anti human IgG1-HRP #ab99774
mouse mAb anti human IgG2-HRP #ab99784
mouse mAb anti human IgG3-HRP #ab99829
mouse mAb anti human IgG4-HRP #ab99817

2) Of the following pairs of goat anti mouse secondary antibodies, which would have the least level of cross-reactivity (ie most specific for its target):

non-pre-adsorbedvs preadsorbed
anti IgM ab97230vs ab98679
anti IgG1 ab98693vsab97240
anti IgG2a ab97245 vs ab98698
anti IgG2b ab97250 vs ab98703
anti IgG2c ab97255 vs ab98722
anti IgG3 ab97260vsab98708


3) What bulk discount can you offer if we wish to purchase antibodies from you with list price totalling over £1000 ?

Many thanks,

Tapan Bhattacharyya
London School of Hygiene & Tropical Medicine

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Answer

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these to you when I receive them.

For the second question, each antibody of each pair is the same with regards to isotype specificity but differ with regards to species specificity. Please note that you have reversed the two anti-IgG1 antibodies in your list: ab98693 is pre-adsorbed for species reactivity, and ab97240 is not.

anti IgM ab97230 vs ab98679 - Same with respect to isotype specificity. (No reactivity with other isotypes was detected). Cross-adsorbed to remove reactivity with human and rat IgG. (Less than 1% reactivity).

anti IgG1 ab98693 vs ab97240 - ab98693 is pre-adsorbed for species reactivity, and ab97240 is not. As above, regarding isotype specificity and species reactivity.

anti IgG2a ab97245 vs ab98698 - As above, regarding isotype specificity and species reactivity.

anti IgG2b ab97250 vs ab98703 - As above, regarding isotype specificity and species reactivity.

anti IgG2c ab97255 vs ab98722 - As above, regarding isotype specificity and species reactivity.

anti IgG3 ab97260 vs ab98708 - As above, regarding isotype specificity and species reactivity.

Our bulk discount is based on number of units purchased, not price. The discount begins at 5% for 5-9 units, 10% for 10-19 units, and so on. A proforma invoice will have the exact amount of the discount, and we will be happy to provide a proforma before you place your order.

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