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Goat Anti-Mouse IgG1 heavy chain (ab97236)

Submit a review Q&A (4)

Key features and details

  • Goat Anti-Mouse IgG1 heavy chain
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, WB, IHC-P, ICC

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase APC APC/Cy7® Biotin FITC Unconjugated

製品の概要

  • 製品名

    Goat Anti-Mouse IgG1 heavy chain
    IgG1 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%.

  • アプリケーション

    適用あり: ELISA, WB, IHC-P, ICCmore details

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab97236の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
1/100 - 1/30000. (Coating ELISA: 1/100 - 1/500, Primary ELISA: 1/1000-1/30000)
WB
1/1000 - 1/30000. (Chemiluminecent: 1/1000 - 1/30000, Colorimetric: 1/1000 - 1/10000)
IHC-P
1/200 - 1/2000.
ICC
Use at an assay dependent dilution.
特記事項
ELISA
1/100 - 1/30000. (Coating ELISA: 1/100 - 1/500, Primary ELISA: 1/1000-1/30000)
WB
1/1000 - 1/30000. (Chemiluminecent: 1/1000 - 1/30000, Colorimetric: 1/1000 - 1/10000)
IHC-P
1/200 - 1/2000.
ICC
Use at an assay dependent dilution.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab97236 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab97236 は論文での使用が確認できていません。

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

I would like to inquire about the availability of the following antibodies:

1. Antibody which can specifically bind to the Fc part of another (primary) monoclonal mouse antibody (isotype IgG1)… (specifically mouse anti-cardiac troponin I).

2. Antibody which can specifically bind to the Fab part of the same primary antibody (IgG1, mouse anti-cardiac troponin I).

Let me know the possibilities or if you have any question.

Regards

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Answer

Thank you for contacting us.

Please check the following datasheets as answer to your first question;

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-Fc-AP-pre-adsorbed-ab49678.html

The antibodies to your second question is

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-ab97240.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-ab97239.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-pre-adsorbed-ab98689.html

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-ab97236.html

For searching the right secondary antibody; please use the secondary search tool on our website.

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Hi, I'll try again all this changes to see if I can improve the quality of the band of Rhodopsin, but what about the size of the band? Is the correct size? And Can you please send me if you have reviews using this ab, Do you have publications with this ab? Thanks,

Read More
Answer

Thank you for contacting us.

The band size seems to be correct. This product has a predicted weight of 39kDa although you band seems a bit smaller this is common when using in real world settings as compared to predictions. Post translational modifications are a common cause of this as well as the inherent inaccuracies of protein marker standards. These are often labeled as 'apparent' size because they may be off by 3-30kDa.
In regards to further data and publications, to our knowledge this product has not been published with. We always include any information that we may have regarding publications, reviews or any information that we about the antibody on our website.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I need help again, I send you the file of my WB with the Rhodopsin ab98887 you report the ab is around 39kDa if you see the image of the wb the band maybe I think it is less than 37kDa, what do you think? And I am very surprise how the ab are up the 50 to 250kDa? Is not very clean ab? What are your suggestions? This is what I did: SDS 10%, transfer to Nitrocellulose, Blocking for 1hr at RT with 5%Milk on TBSt, the primary ab Rhodopsin ab98887 at 1:1000 on 1% Milk overnight, 4 washes with TBSTt every 5 min, incubate with secondary ab98693 at 1:2000 on 1% Milk for 1hr, 6washes of 5min with TBST, and I did ECL the exposition time was very fast like 10 sec, more than that is get it so dark.

Read More
Answer

Thank you for contacting Abcam and thank you for sending the details of your protocol as well as the image. These have been very helpful.

After reviewing your protocol, I would like to suggest a few tips which may help reduce the upper dark areas and increase the band quality for Rhodopsin.
First I would recommend changing your blocking procedures. It may be best for this product if your initial blocking and primary antibody incubation are performed using 3% BSA in TBST. This should be followed by use of a casein based commercial block for incubating your secondary antibody. We have seen that this may dramatically reduce background in western blot. We do carry this type of casein based blocking reagent, ab64234 (https://www.abcam.com/Background-Reducing-Buffer-ab64234-support.html).

Second, you may wish to reduce the concentration of secondary antibody that you use. This product has a large range 1:2000-1:20,000 against which it has been used effectively.

Finally I would suggest increasing the concentration of detergent in your TBST, if you currently use 0.1% Tween20 use 0.25% Tween20 instead.

Read More

Question

Phoned because they have seen no bands in WB with these products.

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this recommended product was not correct for your assay and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number I have also attached a pdf file with our recommended protocol for stripping western blots. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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