Goat Anti-Mouse IgG1 heavy chain (ab9165)

Submit a review Q&A (2)References (2)

Key features and details

  • Goat Anti-Mouse IgG1 heavy chain
  • Host species: Goat
  • Suitable for: ELISA, Immunodiffusion, Immunoelectrophoresis, WB

製品の概要

  • 製品名

    Goat Anti-Mouse IgG1 heavy chain
    IgG1 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    The anti IgG1 only reacts with IgG1 by IEP and gel diffusion analysis when tested against each of the mouse IgG subclasses at 1 mg/ml.

  • アプリケーション

    適用あり: ELISA, Immunodiffusion, Immunoelectrophoresis, WBmore details

  • 免疫原

    Goats were immunized with highly purified mouse IgG1 in Freunds adjuvant

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    The resulting antiserum was sequentially absorbed with IgG2a, IgG2b and IgG3 immunoabsorbants until it was IgG1 specific. The anti IgG1 was then immunoaffinity purified and tested for monospecificity.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (2)

ab9165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9165 は 2 報の論文で使用されています。

  • Fradkin AH  et al. UV photodegradation of murine growth hormone: Chemical analysis and immunogenicity consequences. Eur J Pharm Biopharm N/A:N/A (2014). PubMed: 24758742
  • Fradkin AH  et al. Glass particles as an adjuvant: a model for adverse immunogenicity of therapeutic proteins. J Pharm Sci 100:4953-64 (2011). PubMed: 21721003

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1-2 of 2 Abreviews or Q&A

Question

You sell the following
https://www.abcam.com/Mouse-IgG-secondary-antibody-IgG1-Heavy-Chain-specific-ab9165.html
Is it available labeled for flow cytometery?

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Answer

Thank you for contacting Abcam.

We do not currently supply ab9165 conjugated directly to a fluorphore, but we do have some kits that would allow you to do this yourself. These are our EasyLink conjugation kits:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

Please let me know if there is anything else I can help you with.

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Question

Please send a protocol for using this mouse monoclonal antibody on mouse tissue.

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Answer

Thank you for your enquiry. For staining mouse tissue with mouse monoclonal antibodies, please refer to the following two articles: Fung KM, Messing A, Lee VM, Trojanowski JQ. A novel modification of the avidin-biotin complex method for immunohistochemical studies of transgenic mice with murine monoclonal antibodies. J Histochem Cytochem. 1992 Sep;40(9):1319-28. PMID: 1506669 Hierck BP, Iperen LV, Gittenberger-De Groot AC, Poelmann RE. Modified indirect immunodetection allows study of murine tissue with mouse monoclonal antibodies. J Histochem Cytochem. 1994 Nov;42(11):1499-502. PMID: 7930532 These two references were suggested by the following website: http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/MOMeasy.html This site also mentions a simpler approach, with results illustrated with a set of images: use a secondary antibody that will only recognize the heavy chain of the IgG isotype of the primary. Since each of the isotypes present in mouse is only 20-25% of the total IgG, background due to staining of endogenous IgG will be reduced, compared to using an anti-IgG that recognizes all isotypes. Antibody ab17833 against xanthine oxidase is isotype IgG1. Our only conjugated antibodies specific for IgG1 heavy chain are FITC conjugates, ab48229: https://www.abcam.com/index.html?datasheet=48229 or ab11588: https://www.abcam.com/index.html?datasheet=11588. For light microscopy instead of fluorescent detection, you could use either goat anti-mouse IgG1 ab9165, or rabbit anti-mouse IgG1 ab9172, but these are unconjugated and would need to be followed by a HRP- or AP-conjugated anti-rabbit or anti-goat IgG. I hope this helps. Please do not hesitate to contact me if you have any questions.

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