Goat Anti-Mouse IgG - H&L (SureLight® APC), pre-adsorbed (ab99900)

Thank you for your enquiry and your interest in our products.

This secondary antibody has only been tested for Flow cytometry. However, as long as it is compatible with the primary antibody (host species, isotype), it should be fine for immunofluorescence microscopyas well.

If you need any further assistance in the future, please do not hesitate to contact me.

Vielen Dank für Ihre Anfrage.

Wenn Antikörper mit Biotin gekoppelt werden, wird das meist über freie Anime getan. Das bedeutet das Biotin über den ganzen Antikörper verteilt ist. Die Antikörper werden dann darauf getestet, dass die Bindestelle, die das Zielprotein bindet, nicht von Biotin besetzt ist.

Lange Rede kurzer Sinn: Die Biotin-Moleküle stören sehr wahrscheinlich wenn dann ein Zweitantikörper eingesetzt wird um den primären Antikörper zu binden.

Ich schlage deshalb, wie Sie schon richtig vermuteten, vor einen ungekoppelten primaeren Antikörper für Versuch 2 zu verwenden.

Um den unwahrscheinlichen Fall auszuschließen, dass es sich bei dem sekundären Antikörper um einen fehlerhaften Antikörper handelt, schlage ich aber vor, zuerst den sekundären mit einem anderen primären zu testen. Falls der sekundäre einen anderen angekoppelten primären Antikörper aus der Maus nicht erkennt, werde ich Ihnen den sekundären Antikörper gerne ersetzen.

Ich hoffe, ich konnte Ihnen mit diesen Informationen weiterhelfen. Bitte lassen Sie mich wissen, falls Sie weitere Fragen haben oder der sekundäre Antikörper im Test mit einem un-gekoppelten primären Antiköper nicht funktioniert.

Thank you very much for your feedback.

I am happy to hear that the problem might has been resolved - please keep us updated with the results in FACS as well.

I have looked through your answers, and indeed, with that settings and all the controls, the antibody is expected to work.

I am looking forward to hear back from you and wish you meanwhile a good weekend!

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

In order to understand the problem better and to try to find the origin of the problem, I appreciate if you could confirm the following details. These details will enable us to investigate this case and will provide us with vital information for monitoring product quality.

1.) Do you know whether the primary antibody used is working? Have you tested it with another secondary antibody?

2.) Can you please describe what species and isotype the primary antibody is from?

3.) Can you please provide also details of the dilutions used of the secondary antibody.

4.) Have the filters of the machine be checked to be able to read the APC fluorochrome? What laser and filter have been used?

5.) Howhas the antibody beenstored?

6.)How many times haveyou tested this antibody in total? Have you obtained no results each time?

7.) Finally, can you please also provide the order number of the purchase or the approximate order date with the name of the person ordering? This will help me to locate the order and the vial with its batch number.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I look forward hearing backfrom youand I thank you for your cooperation.