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製品の概要
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製品名Goat Anti-Mouse IgG H&L (HRP)
IgG 二次抗体 製品一覧 -
製品の詳細Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)
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由来種Goat
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ターゲット生物種Mouse
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アプリケーション適用あり: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
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免疫原
mouse IgG whole molecule
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標識HRP
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーpH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
精製度Affinity purified
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特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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特記事項（標識）Horseradish Peroxidase (HRP)
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ポリ/モノポリクローナル
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アイソタイプIgG
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特記事項The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
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研究分野
関連製品
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アプリケーション
Our Abpromise guarantee covers the use of ab6789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/500 - 1/2500. | |
| IP | Use at an assay dependent concentration. PubMed: 17164758 | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/100000. | |
| IHC-P | 1/500 - 1/2500. | |
| IHC-Fr | 1/500 - 1/2500. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/10000. PubMed: 17413034 |
画像
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Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview. -
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.ab49969 Immunoprecipitating HA Tag in human 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789)
参考文献
This product has been referenced in:
- Zhang F et al. Ligand Activation of PPAR? by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1a. Theranostics 8:610-626 (2018). Read more (PubMed: 29344293) »
- Penfornis P et al. Polycomb group protein Suz12 is regulated by a novel miRNA-like small RNA. Sci Rep 8:1720 (2018). Read more (PubMed: 29379063) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"