Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Key features and details
- Goat Anti-Mouse IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (FITC)
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details -
免疫原
mouse whole molecule
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標識
FITC. Ex: 493nm, Em: 528nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
特記事項(標識)
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC (PFA fixed) |
1/1000.
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Immunomicroscopy |
Use at an assay dependent concentration.
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Flow Cyt |
1/500 - 1/2500. PubMed: 17989178
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365.
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ICC/IF | (1) |
1/1000 - 1/5000. (PMID16006199)
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ELISA |
1/10000 - 1/50000.
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WB |
1/10000. PubMed: 17217624
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特記事項 |
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IHC (PFA fixed)
1/1000. |
Immunomicroscopy
Use at an assay dependent concentration. |
Flow Cyt
1/500 - 1/2500. PubMed: 17989178 |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. |
ICC/IF
1/1000 - 1/5000. (PMID16006199) |
ELISA
1/10000 - 1/50000. |
WB
1/10000. PubMed: 17217624 |
画像
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ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (510)
ab6785 は 510 報の論文で使用されています。
- Yu L et al. Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis. Commun Biol 6:133 (2023). PubMed: 36726025
- Sarkis M et al. D2B-Functionalized Gold Nanoparticles: Promising Vehicles for Targeted Drug Delivery to Prostate Cancer. ACS Appl Bio Mater 6:819-827 (2023). PubMed: 36755401
- Li X et al. The members of the miR-148/152 family inhibit cancer stem cell-like properties in gastric cancer via negative regulation of ITGA5. J Transl Med 21:105 (2023). PubMed: 36765401
- Cai J et al. KDM4A, involved in the inflammatory and oxidative stress caused by traumatic brain injury-hemorrhagic shock, partly through the regulation of the microglia M1 polarization. BMC Neurosci 24:17 (2023). PubMed: 36869312
- Kaneko H et al. Zoledronate/Anti-VEGF Neutralizing Antibody Combination Administration Increases Osteal Macrophages in a Murine Model of MRONJ Stage 0-like Lesions. J Clin Med 12:N/A (2023). PubMed: 36902701