Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed (ab96880)
Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
Related conjugates and formulations
製品の概要
-
製品名
Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
-
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cytmore details -
吸着処理血清
Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details -
標識
DyLight® 550. Ex: 562nm, Em: 576nm
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
DyLight® 550 replaces DyLight® 549. -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96880の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/500.
DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
|
ICC/IF |
1/50 - 1/500.
|
|
Flow Cyt |
1/50 - 1/200.
|
特記事項 |
---|
IHC-P
1/50 - 1/500. DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550. |
ICC/IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (10)
ab96880 は 10 報の論文で使用されています。
- Nguyen KDQ et al. Homo-oligomerization of the human adenosine A2A receptor is driven by the intrinsically disordered C-terminus. Elife 10:N/A (2021). PubMed: 34269678
- Mannino G et al. ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells. World J Stem Cells 13:1783-1796 (2021). PubMed: 34909123
- Klepinin A et al. The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins. J Bioenerg Biomembr N/A:N/A (2018). PubMed: 29998379
- Wagener KC et al. Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology. Antioxid Redox Signal 25:41-58 (2016). ICC/IF . PubMed: 27059697
- Dorris ER et al. Pluripotency markers are differentially induced by MEK inhibition in thyroid and melanoma BRAFV600E cell lines. Cancer Biol Ther 17:526-42 (2016). WB . PubMed: 26828826
- Schonenbach NS et al. Adenosine A2a receptors form distinct oligomers in protein detergent complexes. FEBS Lett 590:3295-306 (2016). PubMed: 27543907
- Varikmaa M et al. Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles. Biochim Biophys Acta 1837:232-45 (2014). PubMed: 24189374
- Aldrin-Kirk P et al. Novel AAV-based rat model of forebrain synucleinopathy shows extensive pathologies and progressive loss of cholinergic interneurons. PLoS One 9:e100869 (2014). PubMed: 24999658
- Kaldma A et al. An in situ study of bioenergetic properties of human colorectal cancer: The regulation of mitochondrial respiration and distribution of flux control among the components of ATP synthasome. Int J Biochem Cell Biol 55C:171-186 (2014). IHC-P . PubMed: 25218857
- Varikmaa M et al. Matters of the heart in bioenergetics: mitochondrial fusion into continuous reticulum is not needed for maximal respiratory activity. J Bioenerg Biomembr 45:319-31 (2013). PubMed: 23271420