Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

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製品の概要

  • 製品名

    Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Goat

  • ターゲット生物種

    Mouse

  • 特異性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

     

  • アプリケーション

    適用あり: IHC-P, ICC/IF, Flow Cytmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Horse, Human, Pig, Rabbit, Rat more details

  • 標識

    DyLight® 488. Ex: 493nm, Em: 518nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/1000 - 1/2000.

画像

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)This image is courtesy of an anonymous Abreview.

    ab85137 staining S100 in a human melanoma cell line by Flow Cytometry. The cells were harvested using EDTA and washed in PBS. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at room temperature. A DyLight® 488-conjugated goat anti-mouse IgG H&L (ab96879) (1/100) was used as the secondary antibody.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488, pre-adsorbed) (ab96879) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab2861 (red line).
    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2861, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ab96879 at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

  • Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

  • Zhang Y  et al. Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin avß3. Nanoscale Res Lett 13:281 (2018). Read more (PubMed: 30203331) »
  • Serra M  et al. Evidence of Amniotic Epithelial Cell Differentiation toward Hepatic Sinusoidal Endothelial Cells. Cell Transplant 27:23-30 (2018). Read more (PubMed: 29562778) »
See all 25 Publications for this product

ab96879 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-10 of 13 Abreviews or Q&A

Question

immunogen sequence to BLAST with fish

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Answer

Thank you for contacting us.

I performed a BLAST search with the general term "fish" and only the match below.

poly [ADP-ribose] polymerase 1-like [Takifugu rubripes] 91%

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

These are the antibodies that we have ordered in the recent past that don’t seem to work. My confocal data just shows a lot of background noise. Pl. help.

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Answer

According to my records, ab139923 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.



Looking at our correspondence, it appears that we are awaiting protocol details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.



If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.



We wish you the best of luck with your research and look forward to a reply.

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Question


I am using human PBMCs and human bronchoalveolar cells and I am using flow cytometry for analysing the cells.

I bought recently these antibodies:

The primary ab2779 PPAR alpha antibody [3B6/PPAR]

The secondary Ab DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)


Isotype Control: ab91366 Mouse IgG2b [PLPV219]


Now I would like to expand my experiment and analyse PPAR-delta and PPAR-gamma in the same cells and I will use flow cytometry.

My questions:

1- Which PPAR-delta Ab should I use? My suggestion is PPAR-A ab58137.

2- Can I use the same isotype? That is ab91366 Mouse IgG2b [PLPV219]?

3- Can I use the same secondary Ab? That is DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)

4- Which PPAR-gamma Ab should I use? My suggestion is Ab41928.

5- Can I use the same isotype? That is ab91366 Mouse IgG2b [PLPV219]?

6- Can I use the same secondary Ab? That is DyLight® 488 goat anti-mouse IgG (H+L) (ab96879)

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Answer

Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

1. Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001

2. Alexa Fluor® 488 goat anti-rabbit IgG Cat # A- 11008

3. Cy3® goat anti-mouse IgG (H+L) *2mg/mL Cat A 10521

4. Cy3® goat anti-rabbit IgG (H+L) *2mg/mL A10520

5. Cdc2 p34 200 ìg (PSTAIRE sc-53(Epitope is of human origin

6. Nanog Antibody (A-11) sc-374001

7. Oct-3/4 Antibody (H-134) sc-9081

8. Anti Sox2 Cat. No.sc-17320

9. caspase-3 Antibody (H- 277): sc-7148

10. caspase-2L Antibody (C- 19): sc-626

11. Blasticidin S HCl R210-01 50mg)



Please let us have best alternate products for the above at your earliest.


Sincere regards,

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Answer

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are

https://www.abcam.com/Nanog-antibody-ab70482.html

https://www.abcam.com/Nanog-antibody-ab90640.html

7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

11. We do not sell this chemical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi, I am just starting to use the CD31 antibody to detect the endothelial cells of rabbit vessel sample. We just ordered the Primary Anti-CD31 antibody [JC/70A] (ab9498). I am really new in biology, so please let me ask a stupid question, I guess I can't image the sample with only primary antibody stained, right? What kind of secondary antibody should I use? I find this secondary antibody from related product of the aforesaid primary antibody. Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab96879) Do you think this one is good for my purpose?

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Answer

Thank you for contacting us and asking this question. It does not seem stupid to me at all!

You are correct, you need a secondary antibody that is conjugated to a fluorescent molecule (or an enzyme, followed by the enzyme's substrate to get some color) in order to see where ab9489 is sticking to CD31. The one you picked out, ab96879, is a good choice, assuming you have a microscope that detects fluorescence and has the correct filter for that fluorochrome, DyLight® 488. (It very likely does).

I hope this helps. If you have any other questions, please let us know.

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Question

Wl for the following product code. AB23750 AB11575 AB292 AB6586 AB30394 AB96879 AB96885. If the products does not requires MSDS, please certify the products are non-hazardous products for which no MSDS is provided or required. Thank you.

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Answer

r/>
I can confirm the Safety Data Sheets can be found on the online datasheets. Near the top right hand side of the datasheet, there are two 'buttons', one for Datasheet PDF and the one underneath says SDS. Click on the SDS button to link to the document.

I hope this will be helpful. If you have any difficulties finding these, or if you have any further questions, please do not hesitate to contact me.

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Question

IHC-P
mouse tumor tissue (from lung) with human cell line injected, some muscle tissue attached as well
sees high background for human cellswith priamry Ab as well as isotype ctrl, but also for muscle (does not contain any EGF)
Ab: 1 ug/ml 3h RT
block: horse serum + chicken egg white albumin
AR: none + proteinase K
2nd (ab96879)

suggested: try no primary crtl to determine if problem is secondary

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Answer

Thank you for contacting us.
I am sorry to hear that the antibodies are not working as expected.
As we discussed over the phone, if you please could try the no primary control, this would help us a lot in determining whether the problem comes from the secondary antibody alone, or if there are issues with the primary antibody and isotype control as well.
Please let me know your results.
I look forward to hear back from you and assist you further in resolving his problem.

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Question

The customer want ab13579 with Secondary AB .

Application : WB, IHC both FL & Non FL.

In ab96879 = This is not suitable for WB.

Is it possible to use ab96876 or any one that you can suggest ?

Regards

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Answer

Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC-P, ICC/IF, Flow Cyt, and WB.

It is therefore tested and guaranteed for western blot and immunohistochemistry on paraffin embedded sections. I am sorry I am not sure what you mean by IHC both FL - Non FL, could you clarify?

I hope this will be helpful. Please do not hesitate to contact me if you have any further questions.

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Question

Dear All,



Sending you attached a list of the tendered items. You can see the products at page 3-5. Please give all of the items a check and let us know which items are best available with Abcam.



Early response requested.



Sincere regards,

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Answer

Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

Please find a list of the product code and the links to the respective datasheets below:

ab1012 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=1012 (or use the following: https://www.abcam.com/index.html?datasheet=1012).

ab12209 (Anti-Histone H3 (tri methyl K4) antibody [mAbcam12209] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=12209 (or use the following: https://www.abcam.com/index.html?datasheet=12209).

ab6002 (Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade)

https://www.abcam.com/index.html?datasheet=6002 (or use the following: https://www.abcam.com/index.html?datasheet=6002).

ab124462 (Anti-Flag Tag antibody)

https://www.abcam.com/index.html?datasheet=124462 (or use the following: https://www.abcam.com/index.html?datasheet=124462).

ab6939 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=6939 (or use the following: https://www.abcam.com/index.html?datasheet=6939).

ab96899 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96899 (or use the following: https://www.abcam.com/index.html?datasheet=96899).

ab96919 (Donkey anti-Rabbit IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96919 (or use the following: https://www.abcam.com/index.html?datasheet=96919).

ab96879 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed)

https://www.abcam.com/index.html?datasheet=96879 (or use the following: https://www.abcam.com/index.html?datasheet=96879).

ab96871 (Goat anti-Mouse IgG H&L (DyLight® 488) secondary antibody)

https://www.abcam.com/index.html?datasheet=96871 (or use the following: https://www.abcam.com/index.html?datasheet=96871).

ab97265 (Goat anti-Mouse IgG Fc (HRP) secondary antibody)

https://www.abcam.com/index.html?datasheet=97265 (or use the following: https://www.abcam.com/index.html?datasheet=97265).

ab7481 (Goat Serum)

https://www.abcam.com/index.html?datasheet=7481 (or use the following: https://www.abcam.com/index.html?datasheet=7481).

ab7487 (Rabbit Serum (sterile))

https://www.abcam.com/index.html?datasheet=7487 (or use the following: https://www.abcam.com/index.html?datasheet=7487).

ab46666 (Herring sperm ssDNA)

https://www.abcam.com/index.html?datasheet=46666 (or use the following: https://www.abcam.com/index.html?datasheet=46666).

ab52579 (Ribonuclease A protein (Active))

https://www.abcam.com/index.html?datasheet=52579 (or use the following: https://www.abcam.com/index.html?datasheet=52579).

ab73430 (DNase I protein (Active))

https://www.abcam.com/index.html?datasheet=73430 (or use the following: https://www.abcam.com/index.html?datasheet=73430).

ab64220 (Proteinase K)

https://www.abcam.com/index.html?datasheet=64220 (or use the following: https://www.abcam.com/index.html?datasheet=64220).

ab65621 (Protease Inhibitor Cocktail)

https://www.abcam.com/index.html?datasheet=65621 (or use the following: https://www.abcam.com/index.html?datasheet=65621).

ab116028 (Prism Ultra Protein Ladder (10-245 kDa))

https://www.abcam.com/index.html?datasheet=116028 (or use the following: https://www.abcam.com/index.html?datasheet=116028).


I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Question

Hi!
Thank you for your answer.
Now I have fixed my choice on a primary antibody, I need a secondary.
I have always used Alexa-conjugated secondaries so I have no experience of the DyLight and Chromeo-conjugates that you offer. Both exhibit the same fluorescence spectrum and both come at the same price, so it is hard for me to make a choice. Can you help me? What are the main differences between Dylight and Chromeo?
I think the most important parameter for me would be the resistance to photobleaching because I will use a normal epifluorescence and not a confocal microscope so I will have few control over the exposure of the specimen to light.
Best regards.

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Answer

Thank you for contacting us.

The fluorochromes which are sold under the brand names Alexa, DyLight or Chromeo are designed to ensure an optimal performance and thus are chemically almost identical. As you may have noticed the advantagesfor these include excellent photostability and bright fluorescence (see also flyer attached). So from this point of view, there is not much of a difference between the DyLights or Chromeos.

Therefore, you may want to make your decision based on other factors:

We offer DyLight conjugated antibodies in the wavelengths 488nm, 550nm, 594nm and 650nm (based on the excitation maxima). These fluorochromes are suitable for most settings. We have Chromeo conjugated antibodies in comparable wavelengths available (488nm, 546nm, 642nm), but also for 494nm and 505nm which are somewhere in between. So if you wish to stain your target in ones of these colours (and your microscope has the correct filter settings), I would recommend the Chromeos.

On the other hand, our DyLight antibody range contains a wide variety of antibody formats, such as antibodies against H&L chain (e.g. ab96879), against the Fc part (e.g. ab98736) or F(ab')2 fragments (e.g. ab98739). Also, within our DyLight antibody range we have a number of secondaries available which have been pre-absorbed against other species to minimise potential cross-reactivity (e.g. ab96879). These advantages might be relevant for you depending on the sample type and species and the primary antibody.

Regarding the resistance to photobleaching, I would slightly prefer the DyLight conjugated antibodies, asaccording to customer feedback they seem to perform excellent. However, this is my opinion only and merelybased on our sales volume for the DyLights; we do not have any further data to confirm this.

I hope this information helps. Please do not hesitate to contact us if you need anything further or if I could help you finding the most suitable secondary for your experiments.

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