Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)
Key features and details
- Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
- Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Fr
Related conjugates and formulations
製品の概要
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製品名
Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: ELISA, ICC/IF, Flow Cyt, IHC-P, IHC-Frmore details -
吸着処理血清
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Mouse IgG, whole molecule (H&L)
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標識
Cy5 ®. Ex: 650nm, Em: 667nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis. -
特記事項(標識)
Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 12.6 moles Cy5 per mole of Goat IgG -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species (human labelled with the fluorophore Cy5 here) in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6563の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/10000 - 1/50000. PubMed: 18691532
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ICC/IF | (1) |
1/1000 - 1/5000.
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Flow Cyt |
Use at an assay dependent dilution.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
1/10000 - 1/50000. PubMed: 18691532 |
ICC/IF
1/1000 - 1/5000. |
Flow Cyt
Use at an assay dependent dilution. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)Antanaviciute et al PLoS One. 2014 Jun 19;9(6):e99196. doi: 10.1371/journal.pone.0099196. eCollection 2014. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Formation of TT3s between LSCC cells.
(Panel G) TT4s form between the intersecting rear or secondary lamellipodia establishing functional Cx43 GJs (the arrow in the inset) as confirmed by patch-clamp measurements.Cells were grown in 24-well plates with glass coverslips on the bottom, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.2% Triton X-100/PBS for 3 min. Coverslips were incubated for 1 h with the following primary antibodies: mouse anti-α-tubulin, rabbit anti-Cx43, mouse anti-Cx43, rabbit anti-Cx26, rabbit anti-Cx30, then rinsed with 1% BSA/PBS and incubated with secondary goat anti-mouse IgG H&L (Cy5) (ab6563, Abcam Cambridge, UK) or with donkey anti-rabbit IgG (FITC) for 30 min. The F-actin network was visualized using Alexa Fluor 594 phalloidin; coverslips were incubated with the dye for 30 min at 37°C. Coverglasses were attached using Vectashield Mounting Medium with DAPI and sealed with clear nail polish. MitoTracker Green was used to stain mitochondria in live cells.
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Immunocytochemistry - Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)This image is courtesy of an Abreview submitted by Dr laurent barbeab2792 (PDI antibody (RL90) - ER marker) staining human U2-OS (bone osteosarcoma) cells by ICC/IF. ab6563 (undiluted) was used as the secondary.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab6563 は 54 報の論文で使用されています。
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- Li X et al. Ethacridine inhibits SARS-CoV-2 by inactivating viral particles in cellular models. bioRxiv N/A:N/A (2020). PubMed: 33140048
- Huang H & Lennikov A CXCR5/NRF2 double knockout mice develop retinal degeneration phenotype at early adult age. Exp Eye Res 196:108061 (2020). PubMed: 32387618